FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001602481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365186
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC936963.96146434149365Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC814993.4457755119470517Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAT604272.554851922850888TruSeq Adapter, Index 3 (97% over 36bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA503472.1286697959483947TruSeq Adapter, Index 7 (97% over 35bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT461861.9527428286823953Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG336421.4223828485370706Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGC308381.3038298045058614TruSeq Adapter, Index 3 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA276401.1686184511492965Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG175510.7420558044906405Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA146540.6195707229790807TruSeq Adapter, Index 3 (97% over 36bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA132860.5617317200423139TruSeq Adapter, Index 3 (97% over 36bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC101370.4285920853581917Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG98890.41810665207725733TruSeq Adapter, Index 3 (97% over 36bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA90350.381999555214685Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATG88700.37502335968503114TruSeq Adapter, Index 3 (97% over 36bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG79170.33473054550466647Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG77170.3262745509232678Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG73520.31084236081221517Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC73110.3091088819230285Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA68880.2912244533833703Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT56830.24027708603044326Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT56150.23740204787276775Clontech SMART CDS Primer II A (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC56070.23706380808951177Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA53900.22788905396869422Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT53600.2266206547814844Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT53270.22522541567555365Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT51660.2184183400375277Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC51070.21592382163601512Clontech Universal Primer Mix Long (96% over 25bp)
TATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT48080.2032821097368241Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC46600.19702467374658905Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC46070.1947838351825184Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA45830.19376911583275058Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC45180.19102091759379602Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC44440.1878921995986785Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG42120.17808324588425603Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC40200.1699654910861133Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT40040.16928901151960143Clontech SMART CDS Primer II A (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC39900.1686970918989035Clontech Universal Primer Mix Long (96% over 25bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA38320.16201685617959855Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA36240.15322262181494395Clontech Universal Primer Mix Long (96% over 25bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG34640.146457826149825Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG34090.14413242763994036Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30930.13077195620133047Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC30300.12810831790818988Clontech Universal Primer Mix Long (96% over 26bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG29780.12590975931702622Clontech Universal Primer Mix Long (96% over 25bp)
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGC29520.1248104800214444TruSeq Adapter, Index 3 (97% over 36bp)
ATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA29510.1247682000485374Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA29340.12404944050911852Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC28920.1222736816470248Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG28920.1222736816470248Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT28610.120963002486908Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA27220.11508608625283594Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC27170.11487468638830096Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG26370.11149228855574149Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC25910.1095474098020198Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG25810.10912461007294987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA25510.10785621088574007Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG24820.10493889275515753Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24650.10422013321573864Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA23920.10113369519352813Clontech SMART CDS Primer II A (96% over 26bp)
TTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGAT23880.10096457530190016Clontech SMART CDS Primer II A (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)