FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001602647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275425
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC804956.311229590136621Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC777466.095693592331967Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA261482.0501401493619773TruSeq Adapter, Index 7 (97% over 35bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT170611.3376717564733325Clontech SMART CDS Primer II A (100% over 26bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG168941.3245780818158652Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA167541.3136013485700844TruSeq Adapter, Index 7 (97% over 37bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC102640.8047513573906737Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT99200.7777799557010409TruSeq Adapter, Index 7 (97% over 38bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG71180.5580884803104847Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT59930.4698825881568888Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT59140.4636885743967697Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG49490.3880275202383519TruSeq Adapter, Index 7 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG49330.38677303643883415Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG46140.3617617656859478TruSeq Adapter, Index 7 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA40240.31550267557872863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA38220.29966481760981634Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT32180.2523080541780191TruSeq Adapter, Index 7 (97% over 38bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT28170.22086755395260405TruSeq Adapter, Index 7 (97% over 38bp)
ATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG23180.1817433404551424Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA19460.15257659211635338Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG17990.14105102220828353Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG17980.14097261697081365Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC17820.13971813317129586Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA14580.11431483623106024Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG14320.1122763000568438Clontech SMART CDS Primer II A (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)