FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001602648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275425
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG754975.919360213262246Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT749985.880235999764785Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCA245261.9229668541858596Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGA162591.2747907560225025Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG160691.2598937609032284Illumina Single End PCR Primer 1 (100% over 30bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG101500.7958131603191093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGC97370.763431797244056Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT96870.7595115353705628Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG93490.7330105651057491Illumina Single End PCR Primer 1 (100% over 28bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG72930.5718093968677108Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA57120.4478507164278574Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA52410.41092184957955186Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAG47760.3744634141560656Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAG43000.3371425211204108Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG33470.26242232981163144Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAG31190.24454593566850266Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC27110.21255659878079855Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAG26370.20675461120802868Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTC21680.16998255483466296Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT18540.14536331026912597Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTC17150.13446498226081502Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA14740.11556932003057804Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG13580.10647431248407395Illumina Single End PCR Primer 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)