FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001602661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922835
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC298351.5516151931913036Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC242891.2631869089131413Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT225591.1732155905212875Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG165860.8625805126284888Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT163320.8493708508530373Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT107500.5590703310476458TruSeq Adapter, Index 1 (100% over 35bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC76470.3976940299089625TruSeq Adapter, Index 13 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA75010.39010107471519917Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG65310.33965472856485346Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC57340.2982055142536931Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAG47060.24474278864281127TruSeq Adapter, Index 13 (97% over 38bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG46160.24006219982473798Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG27060.14072970379673763No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG24230.12601185229101822Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC23080.12003109991236899Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAG22210.11550653072156478No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCC21870.11373830827918151No Hit
ATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGT21800.11337426248222027No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA21730.11301021668525901No Hit
ATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCT21020.10931775217322338No Hit
ATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATA20950.10895370637626213No Hit
GTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAA20870.10853765403687785No Hit
ATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCG20440.10630137271268726No Hit
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT20380.10598933345814905TruSeq Adapter, Index 8 (97% over 36bp)
ATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGA20290.1055212745763417No Hit
ATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGA20110.10458515681272705No Hit
GTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAG20040.10422111101576578No Hit
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA19940.10370104559153542Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGT19520.10151677080976788No Hit
ATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATG19330.10052864650373017No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)