FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001602662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922835
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG258841.346137344077885Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT243561.2666713472554847Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG233241.2130005954749108Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG215021.1182446751801376Illumina Single End PCR Primer 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA95710.49775461753088535Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCC92480.48095650432824455Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG88250.4589577368832999Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC83910.43638689747170195Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTC65370.33996676781939167Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG41240.21447498095260384Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTA39000.2028255154498436Illumina Single End PCR Primer 1 (96% over 33bp)
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG22780.11847090363967788No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG21720.11295821014283597Clontech SMART CDS Primer II A (96% over 26bp)
ATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGT21580.11223011854891345No Hit
GTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAG21440.11150202695499092No Hit
ATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATA21430.11145002041256791No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCC20540.10682143813691763No Hit
ATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCG20110.10458515681272705No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19800.1029729539976129No Hit
ATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATG19680.10234887548853647No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG19570.10177680352188305Illumina Single End PCR Primer 1 (100% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)