FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001602747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574371
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT219311.3930007603036387Clontech SMART CDS Primer II A (100% over 26bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC162361.0312689956814498Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC150680.95708063728308Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG125080.794476016135968Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT105440.669727783349668Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC66380.42162870124005075Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA57320.36408190953720565Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT56790.3607154857400193TruSeq Adapter, Index 1 (100% over 35bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG43480.2761737862295482Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTC41230.2618823644490403TruSeq Adapter, Index 13 (97% over 37bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG31440.19969880034629703Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAG26600.16895636416067117TruSeq Adapter, Index 13 (97% over 38bp)
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG20010.1270983777013169No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC18660.11852352463301216Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG17620.11191771189891075Clontech SMART CDS Primer II A (96% over 26bp)
GTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAG17170.10905942754280916No Hit
ATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATA16990.10791611380036853No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16590.10537541659494491No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCC16480.1046767248634534No Hit
ATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGA16450.10448617257304664No Hit
ATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCT16410.10423210285250427No Hit
ATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGT15930.10118326620599592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)