FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001602748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574371
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG189811.2056243414036463Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA158781.0085297556929085Illumina Single End PCR Primer 1 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT157420.9998913851944682Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG144480.9176998305990138Illumina Single End PCR Primer 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC96240.6112917476249244Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA72780.4622798565268288Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG55880.3549353995976806Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGG55820.354554295016867Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT41150.26137422500795554Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG29440.18699531431917887Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG26440.1679400852785017Illumina Single End PCR Primer 1 (97% over 35bp)
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG17730.11261640363040223No Hit
ATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATA17010.1080431486606397No Hit
GTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAG16620.10556596888535168No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG16510.10486727715386017Illumina Single End PCR Primer 1 (100% over 28bp)
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCC16030.10181844050735182No Hit
ATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGT15920.10111974877586032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)