FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001602905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016663
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA99530.49353808742462174Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGGGATCAAGTGAATAAGTGCATATGGTGGATGCCTAGGCGAT78850.39099244643254727No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC76020.37695936306661054Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT74530.3695709198810114Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA73460.36426512511014486Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTATATATTCAGGAGGAGTTACCCTTGCGGGTGGGTTTCCCCATTCGG69960.3469097216540394No Hit
TATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTAGCT69560.3449262469733416No Hit
ACTTAGATGTTTCAGTTCACTCGGTTTGCCACAACCTCCCTATATATTCA53310.26434758806999487No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA52840.26201700532017497Clontech Universal Primer Mix Long (96% over 25bp)
ATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTAGCTC49000.2429756483854764No Hit
CTCTATGGCCGGACTTTCCATTCCGTTCTACTATCGCATATGCTAAAAAT48640.24119052117284845No Hit
GTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGT48250.23925663335916808No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT46770.23191777704058636Clontech Universal Primer Mix Long (96% over 26bp)
GTTCCATGTTCGCTCGCCACTACTTACGGAATCTCGGTTGATTTCTTTTC44560.2209590794297312No Hit
GTGGTAGCCTGCGTAAAGTTTCGGGGAGCTGGCAAACAAGCTTTGATCCG43800.21719047753640544No Hit
GAATATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTA42430.2103970767550156No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG40910.20285987296836408Clontech SMART CDS Primer II A (96% over 26bp)
GTATAAGGGGCTATCACCCTCTATGGCCGGACTTTCCATTCCGTTCTACT38290.1898681138097937No Hit
CCCTAGTTCCACACACGGGATTTCGTATAAGGGGCTATCACCCTCTATGG36430.1806449565445491No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG36400.18049619594349675Clontech Universal Primer Mix Long (96% over 26bp)
CCTGAATATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAA34680.17196725481649636No Hit
GTATCAACGCAGAGTACATGGGGGATCAAGTGAATAAGTGCATATGGTGG32530.16130607840774586No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC32170.15952095119511786Clontech SMART CDS Primer II A (96% over 26bp)
GAATAAGTGCATATGGTGGATGCCTAGGCGATTACAGGCGATGAAGGACG31990.15862838758880388No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30950.15347135341898968Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG30670.15208292114250124Clontech Universal Primer Mix Long (96% over 26bp)
GTGAATAAGTGCATATGGTGGATGCCTAGGCGATTACAGGCGATGAAGGA29950.14851266671724528No Hit
CATCTAAGTAGCTCGAGGAAAAGAAATCAACCGAGATTCCGTAAGTAGTG29920.14836390611619293No Hit
ACTTACGGAATCTCGGTTGATTTCTTTTCCTCGAGCTACTTAGATGTTTC29180.14469447795690207No Hit
ATATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTAGC29150.14454571735584976No Hit
GTGCATATGGTGGATGCCTAGGCGATTACAGGCGATGAAGGACGTGGTAG29040.14400026181865785No Hit
ACATGGAACAGCCTGTTATTTTTAGCATATGCGATAGTAGAACGGAATGG28950.14355398001550085No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC28800.1428101770102392Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCTATATATTCAGGAGGAGTTACCCTTGCGGGTGGGTTTCCCCATTCG28550.1415705053348031No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC28240.1400333124572623Clontech Universal Primer Mix Long (96% over 26bp)
GTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCC28230.13998372559024486No Hit
CTACTATCGCATATGCTAAAAATAACAGGCTGTTCCATGTTCGCTCGCCA27160.13467793081937834No Hit
TCCGTAAGTAGTGGCGAGCGAACATGGAACAGCCTGTTATTTTTAGCATA26390.13085974205903514No Hit
ATCACCCTCTATGGCCGGACTTTCCATTCCGTTCTACTATCGCATATGCT25380.1258514684902733No Hit
GCATATGGTGGATGCCTAGGCGATTACAGGCGATGAAGGACGTGGTAGCC25370.12580188162325584No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG25050.12421510187869762Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC24920.12357047260747087Clontech SMART CDS Primer II A (96% over 26bp)
ACTATCGCATATGCTAAAAATAACAGGCTGTTCCATGTTCGCTCGCCACT24660.12228121406501731No Hit
GCCTTGGAGGATGGTCCCCCCATGTTCAGACAAGGTTTCTCGTGCCCCGC24600.12198369286291265No Hit
GTGGATGCCTAGGCGATTACAGGCGATGAAGGACGTGGTAGCCTGCGTAA24580.12188451912887777No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23930.11866137277274388Clontech Universal Primer Mix Long (96% over 25bp)
ATATTCAGGAGGAGTTACCCTTGCGGGTGGGTTTCCCCATTCGGACATTT23090.11449607594327857No Hit
GTATAAAGGTACAGGAATATTAACCTGTTTCCCATCAGCTACGCATCTCT22480.11147127705521448No Hit
GTGAATACATAGCTATGCGAGGCGAACCGGGTGAACTGAAACATCTCAGT22410.11112416898609238No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA22340.11077706091697026Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21890.10854565190118526Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACATAGCTATGCGAGGCGAACCGGGTGAACTGAAACATCTCAGTAGCT21850.10834730443311551No Hit
CCTCCTGAATATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATC21090.10457870253978974No Hit
TTTCCAGACTGTTCTGCTAAGAATCTCGCTATCACTAACAGGCTTTTCCG20890.10358696519944086No Hit
TATCAACGCAGAGTACATGGGGGATCAAGTGAATAAGTGCATATGGTGGA20450.10140514305067332No Hit
ATGAAGGACGTGGTAGCCTGCGTAAAGTTTCGGGGAGCTGGCAAACAAGC20380.10105803498155122No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)