FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001602906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016663
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA100810.4998852064028546Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGGGATCAAGTGAATAAGTGCATATGGTGGATGCCTAGGCGAT89760.44509171834857875No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA77940.3864800415339598Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC77010.38186846290133747Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT72730.3606452838178714Clontech Universal Primer Mix Long (96% over 26bp)
CCCTATATATTCAGGAGGAGTTACCCTTGCGGGTGGGTTTCCCCATTCGG68110.3377361512558122No Hit
TATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTAGCT67740.3359014371761668No Hit
GTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGT55350.27446330894155346No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA52910.2623641133892971Clontech Universal Primer Mix Long (96% over 25bp)
CTCTATGGCCGGACTTTCCATTCCGTTCTACTATCGCATATGCTAAAAAT51830.2570087317514131No Hit
ACTTAGATGTTTCAGTTCACTCGGTTTGCCACAACCTCCCTATATATTCA50400.24991780976791858No Hit
ATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTAGCTC48200.23900869902408087No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT46710.2316202558384817Clontech Universal Primer Mix Long (96% over 26bp)
GTTCCATGTTCGCTCGCCACTACTTACGGAATCTCGGTTGATTTCTTTTC45890.22755413274305128No Hit
GTGGTAGCCTGCGTAAAGTTTCGGGGAGCTGGCAAACAAGCTTTGATCCG43590.2161491533290391No Hit
GAATATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTA43230.21436402611641112No Hit
GTATAAGGGGCTATCACCCTCTATGGCCGGACTTTCCATTCCGTTCTACT41940.2079673202711608No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG41190.2042483052448525Clontech SMART CDS Primer II A (96% over 26bp)
CCCTAGTTCCACACACGGGATTTCGTATAAGGGGCTATCACCCTCTATGG38320.19001687441084605No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG35940.17821520006069433Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGGGATCAAGTGAATAAGTGCATATGGTGG34630.17171932048140914No Hit
CCTGAATATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAA33200.16462839849791464No Hit
GTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCC32500.16115731780669354No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG32480.16105814407265864Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAATAAGTGCATATGGTGGATGCCTAGGCGATTACAGGCGATGAAGGACG31740.15738871591336778No Hit
GTGCATATGGTGGATGCCTAGGCGATTACAGGCGATGAAGGACGTGGTAG30940.15342176655197226No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG30380.15064490199899536Clontech Universal Primer Mix Long (96% over 26bp)
ACTTACGGAATCTCGGTTGATTTCTTTTCCTCGAGCTACTTAGATGTTTC29850.14801679804707082No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC29460.1460829102333905Clontech SMART CDS Primer II A (96% over 26bp)
CATCTAAGTAGCTCGAGGAAAAGAAATCAACCGAGATTCCGTAAGTAGTG29330.1454382809621637No Hit
ACATGGAACAGCCTGTTATTTTTAGCATATGCGATAGTAGAACGGAATGG28770.14266141640918686No Hit
ATATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTAGC28640.1420167871379601No Hit
GTGAATAAGTGCATATGGTGGATGCCTAGGCGATTACAGGCGATGAAGGA28240.1400333124572623No Hit
CTACTATCGCATATGCTAAAAATAACAGGCTGTTCCATGTTCGCTCGCCA27890.13829777211165178No Hit
GCCTTGGAGGATGGTCCCCCCATGTTCAGACAAGGTTTCTCGTGCCCCGC27760.137653142840425No Hit
TCCCTATATATTCAGGAGGAGTTACCCTTGCGGGTGGGTTTCCCCATTCG27590.13681016610112845No Hit
ATCACCCTCTATGGCCGGACTTTCCATTCCGTTCTACTATCGCATATGCT27300.1353721469576226No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC27210.13492586515446559Clontech Universal Primer Mix Long (96% over 26bp)
GCATATGGTGGATGCCTAGGCGATTACAGGCGATGAAGGACGTGGTAGCC26500.13140519759622704No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG26310.1304630471228956Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC26050.12917378858044204Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTATCGCATATGCTAAAAATAACAGGCTGTTCCATGTTCGCTCGCCACT25100.12446303621378486No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC23970.11885972024081368Clontech SMART CDS Primer II A (96% over 26bp)
GTGGATGCCTAGGCGATTACAGGCGATGAAGGACGTGGTAGCCTGCGTAA23880.11841343843765667No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23470.11638037688994146Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT23270.11538863954959258Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTCAGGAGGAGTTACCCTTGCGGGTGGGTTTCCCCATTCGGACATTT22970.11390103353906925No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA22940.1137522729380169Clontech SMART CDS Primer II A (96% over 26bp)
GTATAAAGGTACAGGAATATTAACCTGTTTCCCATCAGCTACGCATCTCT22190.11003325791170859No Hit
TATCAACGCAGAGTACATGGGGGATCAAGTGAATAAGTGCATATGGTGGA22180.10998367104469114No Hit
GTGAATACATAGCTATGCGAGGCGAACCGGGTGAACTGAAACATCTCAGT22170.10993408417767371No Hit
TTTCCAGACTGTTCTGCTAAGAATCTCGCTATCACTAACAGGCTTTTCCG21780.10800019636399338No Hit
CCTCCTGAATATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATC20480.10155390365172565No Hit
TACTTATACAGGGCTATCACCTTCTTTGGCTCTACTTTCCAGTAGATTCT20350.10090927438049888No Hit
ATACATAGCTATGCGAGGCGAACCGGGTGAACTGAAACATCTCAGTAGCT20190.10011588450821976No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)