FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001602918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129595
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25370.22459377033361513Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACA17940.1588179834365414No Hit
ATTCTAAGGCGCTTGAGAGAACTCGGGAGAAGGAACTCGGCAAATTGGTA16970.15023083494526798No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC16080.14235190488626456Clontech Universal Primer Mix Long (96% over 25bp)
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT15110.13376475639499114No Hit
TTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATCGCGGAAGGTTGT15080.1334991744828899No Hit
GTACATGGGGTCTTTCCGTCTAGCCGCGGGTAGATTGCATCATCACAAAC14990.13270242874658617No Hit
GTATCAACGCAGAGTACATGGGGTCTTTCCGTCTAGCCGCGGGTAGATTG13850.12261031608673904No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA13800.12216767956657032Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13310.11782984166891673Clontech Universal Primer Mix Long (96% over 26bp)
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCGTGTTTGC12540.11101323925831826No Hit
TCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCT11990.10614423753646218No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTCAATCAAG11600.10269167267914607No Hit
CTACTAGGGCGTACCTTATCCCGAAGTTACGGTACCAATTTGCCGAGTTC11590.10260314537511232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)