FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001602955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972233
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC421702.1381854983665725Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC382571.9397809488027025Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT261511.3259589510975631Clontech SMART CDS Primer II A (100% over 26bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA154690.7843393757228482TruSeq Adapter, Index 7 (97% over 35bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT142770.7239002693900771Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGA113740.5767067075746121TruSeq Adapter, Index 7 (97% over 37bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG90980.4613045213217708Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG81710.41430196127942287Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATT71090.36045436822119903TruSeq Adapter, Index 7 (97% over 38bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC43300.21954809599068673Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG31460.15951462124404167TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG30440.1543428185209354TruSeq Adapter, Index 7 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC29560.1498808710735496Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT24660.12503593642333335Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAT21930.11119375854678427TruSeq Adapter, Index 7 (97% over 38bp)
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG21870.11088953485718979No Hit
GTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAG20690.10490646896183159No Hit
GTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAG20000.101407896531495No Hit
ATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCC19760.10019100177311707No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)