FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001602956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972233
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA420782.1335207351261234Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG377261.9128571522735904Illumina Single End PCR Primer 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT218091.1058024077276873Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGG153820.7799281322237281Illumina Single End PCR Primer 1 (97% over 35bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG128110.6495682812324913Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGAT115200.5841094840214113Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG78340.397214730713866Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAG72130.36572757884083673Illumina Single End PCR Primer 1 (97% over 35bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC41410.20996504976846042Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG39850.20205523383900378Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGA31760.1610357396920141Illumina Single End PCR Primer 1 (97% over 35bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGA30340.15383577903827794Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATA22400.11357684411527443Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA21490.10896278482309138Illumina Single End PCR Primer 1 (100% over 26bp)
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG19900.10090085704883753No Hit
ATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGT19760.10019100177311707No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)