FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001602977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001602977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250504
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT236459.438971034394662Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG227339.07490499153706Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA135715.417478363618945Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT42421.6933861335547535Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36111.441493948200428Clontech SMART CDS Primer II A (100% over 24bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34221.3660460511608596Clontech SMART CDS Primer II A (100% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31281.2486826557659758No Hit
TAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT27501.0977868616868394Clontech SMART CDS Primer II A (100% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT27331.0910005429055025Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT26071.0407019448791237Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC22840.9117618880337239Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC22220.8870117842429661Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20950.8363139909941558Clontech Universal Primer Mix Long (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20000.7983904448631559Clontech SMART CDS Primer II A (100% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA19000.758470922619998Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA18840.7520837990610928Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC17710.7069747389263246Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA17300.6906077348066298Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC17060.681027049468272Clontech SMART CDS Primer II A (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT17010.679031073356114Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT16460.6570753361223772Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG16400.6546801647877879Clontech SMART CDS Primer II A (96% over 26bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC16210.6470954555615878Clontech SMART CDS Primer II A (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA15370.6135630568773353Clontech SMART CDS Primer II A (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG15250.6087727142081564Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC13910.5552805544023249Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA13320.5317280362788618Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC12640.5045827611535145Clontech SMART CDS Primer II A (96% over 26bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT12620.5037843707086513Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC12600.5029859802637882Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC12400.4950020758151566Clontech Universal Primer Mix Long (96% over 25bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.48901414747868294Clontech SMART CDS Primer II A (100% over 22bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC11810.4714495576916935Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTCCGGAATTCATGTATACAG11580.46226806757576727Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG10730.4283364736690831Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG10120.40398556510075684Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT9900.39520327020726215Clontech Universal Primer Mix Long (96% over 25bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG9720.38801775620349377Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCT9430.376441094752978Clontech SMART CDS Primer II A (96% over 26bp)
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9270.37005397119407274Clontech SMART CDS Primer II A (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC9210.3676587998594833Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTA9080.36246926196787277Clontech SMART CDS Primer II A (96% over 26bp)
CGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT8840.3528885766295149Clontech SMART CDS Primer II A (96% over 26bp)
TGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC8760.34969501485006227Clontech SMART CDS Primer II A (96% over 26bp)
CACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC8670.3461022578481781Clontech SMART CDS Primer II A (96% over 26bp)
TAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA8200.32734008239389395Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG7910.3157634209433781Clontech Universal Primer Mix Long (96% over 26bp)
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT7410.29580365982179924Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC7370.2942068789320729Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA7040.28103343659183083Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT6970.2782390700348098Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.27544470347778877Clontech SMART CDS Primer II A (100% over 21bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT6810.2718519464759046Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA6390.2550857471337783Clontech SMART CDS Primer II A (96% over 26bp)
TAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG6380.2546865519113467Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT6290.25109379490946254Clontech SMART CDS Primer II A (96% over 25bp)
TGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCC6050.24151310957110464Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT5980.2387187430140836Clontech Universal Primer Mix Long (100% over 23bp)
TCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT5880.23472679078976783Clontech SMART CDS Primer II A (96% over 25bp)
ACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG5480.2187589818925047Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG5440.2171622010027784Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG5420.21636381055791526Clontech SMART CDS Primer II A (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC5280.21077507744387317Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA5270.21037588222144157Clontech SMART CDS Primer II A (96% over 26bp)
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT5190.20718232044198895Clontech Universal Primer Mix Long (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4950.19760163510363107Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG4940.19720243988119948Clontech Universal Primer Mix Long (96% over 26bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAAGCAGTGGTATCAACG4780.19081531632229426Illumina Single End PCR Primer 1 (100% over 33bp)
TGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT4760.1900169258774311Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC4760.1900169258774311Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA4760.1900169258774311Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCAC4600.18362980231852585Clontech SMART CDS Primer II A (96% over 26bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4540.18123463098393638Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGT4090.16327084597451538Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT4050.16167406508478907Clontech SMART CDS Primer II A (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA3960.15808130808290488Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCACACGTC3960.15808130808290488Clontech SMART CDS Primer II A (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA3960.15808130808290488Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA3920.15648452719317854Clontech Universal Primer Mix Long (96% over 26bp)
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG3860.15408935585858907Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG3770.15049659885670488Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATCAACGCAGA3670.1465046466323891Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT3640.14530706096509438Clontech SMART CDS Primer II A (100% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG3570.14251269440807332Clontech Universal Primer Mix Long (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG3510.14011752307348385Clontech Universal Primer Mix Long (95% over 24bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG3440.13732315651646282Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3410.13612557084916807Clontech Universal Primer Mix Long (96% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG3380.13492798518187335Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG3370.13452878995944179Clontech Universal Primer Mix Long (96% over 25bp)
TCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT3360.1341295947370102Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCACACGTCTGAACTC3270.130536837735126Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA3240.12933925206783126Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3230.12894005684539966Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT3110.12414971417622075Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT3110.12414971417622075Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.12015776195190497No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCA3010.12015776195190497Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC2900.11576661450515761Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2840.11337144317056813Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTATCAACGCAGA2810.11217385750327341Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC2780.11097627183597866Clontech Universal Primer Mix Long (96% over 25bp)
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA2760.11017788139111552Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.10898029572382077No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATCGGAAGAGCACACGTC2710.10818190527895763Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC2680.10698431961166288Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2680.10698431961166288Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCA2640.10538753872193658Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT2630.10498834349950499Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCCCTAACTCCCCCCCATATT2610.10418995305464185Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG2550.10179478172005238Clontech Universal Primer Mix Long (96% over 25bp)

[FAIL]Adapter Content

Adapter graph