FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001603005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001603005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117857
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1705914.47432057493403Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1473812.504984854527097Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT71826.093825568273416TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG66955.680612946197511TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA63305.370915601109819TruSeq Adapter, Index 7 (97% over 37bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG48224.091398898665332Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCT36823.1241249989393927TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC24922.1144268053658246Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA18191.5433958101767395TruSeq Adapter, Index 19 (97% over 38bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG17631.4958806010673953TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA16991.4415775049424302TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG16151.3703046912784136TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT12811.0869104083762526Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGC8690.7373342270717903TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGC8120.6889705320854934TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA7210.6117583172828088Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC6970.591394656235947Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC5720.48533392161687466Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5220.4429096277692458Clontech SMART CDS Primer II A (100% over 26bp)
ATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4970.4216974808454313Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG3690.31309128859550134Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2860.24266696080843733Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2840.24096998905453218Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA2560.21721238449986Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG2370.20109115283776102Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA2280.1934547799451878Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGT2130.18072749179089914Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC2100.1781820341600414No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG1940.16460626012880017Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA1890.16036383074403726Clontech Universal Primer Mix Long (96% over 26bp)
ATATTTATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1800.15272745785146405Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATTTATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1490.12642439566593414Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA1410.11963650865031351Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1280.10860619224993Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1240.1052122487421197Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC1180.10012133348040422Clontech Universal Primer Mix Long (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)