FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001603006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001603006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117857
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT1703914.45735085739498Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1446912.276742153626854Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAA71726.085340709503891Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATT67255.706067522506088Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGA64255.451521759420315Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG45843.889459259950618Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAG37203.156367462263591Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG23041.95491146049874Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT18441.5646079571005542Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAG18121.5374564090380716Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGAT17031.4449714484502405Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAG15791.3397591997081209Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA11410.9681223856028918Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTA8850.7509100011030316Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTA8090.6864250744546357Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTA6940.5888491986050892Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG6160.5226673002027882Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTA5670.48109149223211184Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT5030.4267883961071468Illumina Single End PCR Primer 1 (100% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4310.3656974129665612Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3880.32921252025760034Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT2830.2401215031775796Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG2820.23927301730062703Illumina Single End PCR Primer 1 (100% over 22bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG2360.20024266696080845Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA2340.1985456952069033Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA2090.17733354828308884Clontech Universal Primer Mix Long (96% over 26bp)
TATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT2070.17563657652918369Illumina Single End PCR Primer 1 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG2060.1747880906522311Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG1700.14424259908193826Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT1520.12896985329679186No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1450.12303045215812383Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT1240.1052122487421197Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1220.10351527698821454Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)