FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001603015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001603015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126396
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1840314.559796196082154Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1633912.926833127630621Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT80866.397354346656539TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGA78186.185322320326592TruSeq Adapter, Index 7 (97% over 37bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAG67975.377543593151682TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG52254.133833349156619Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC28362.2437418905661572Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA22661.792778252476344TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAA21141.6725212823190605TruSeq Adapter, Index 19 (97% over 38bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG20971.6590714896041014TruSeq Adapter, Index 7 (97% over 36bp)
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCT20531.6242602614006771TruSeq Adapter, Index 19 (97% over 38bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG20221.5997341688028102TruSeq Adapter, Index 7 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15991.265071679483528Clontech SMART CDS Primer II A (100% over 26bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT13831.0941801955758093Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA8210.6495458717048008Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC8060.6376784075445426Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC6950.5498591727586316Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6530.5166302731099085Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGC6340.501598151840248TruSeq Adapter, Index 19 (97% over 38bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5510.4359315168201526Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGC5360.4240640526598943TruSeq Adapter, Index 19 (97% over 38bp)
TATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4000.31646571094021964Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.3156745466628691No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG3950.31250988955346687Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG3420.2705781828538878Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3260.25791955441627895Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA3110.24605209025602073Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2850.2254818190449065No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2710.2144055191619988No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC2680.21203202632994714No Hit
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA2480.19620874078293618Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG2260.17880312668122408Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGT2180.1724738124624197Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2160.1708914839077186Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.16693566252096584No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2080.1645621696889142Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.14873888414190323Clontech SMART CDS Primer II A (100% over 21bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.14715655558720214No Hit
ATATTTATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1860.14715655558720214Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1740.13766258425899552Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1700.13449792714959335Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC1680.13291559859489224Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1560.12342162726668565Clontech Universal Primer Mix Long (96% over 26bp)
ATATTTATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1520.12025697015728347Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1470.11630114877053072No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.11471882021582962No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA1400.11076299882907686Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA1380.10918067027437578Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT1370.10838950599702521Clontech SMART CDS Primer II A (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC1340.10601601316497357Clontech Universal Primer Mix Long (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)