FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001603016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001603016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126396
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG1790414.165005221684229Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1579512.496439760751922Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTC75926.006519193645368Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG75635.983575429602203Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA64345.090350960473432Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG49443.911516187221115Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG25652.0293363714041583Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT21711.7176176461280421Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCA20331.608436975853666Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACG20171.5957783474160574Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT19541.5459349979429728Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCA18671.477103705813475Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA11840.9367385043830501Illumina Single End PCR Primer 1 (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG7920.6266021076616348Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGT7360.5822969081300041Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG6910.5466945156492294Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGT6610.522959587328713Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT6110.48340137346118545Illumina Single End PCR Primer 1 (100% over 33bp)
ATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTAC6080.4810278806291339Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTAC4830.3821323459603152Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4620.36551789613595365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT3830.3030159182252603Illumina Single End PCR Primer 1 (100% over 33bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG3530.2792809899047438Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG3100.24526092597867022Illumina Single End PCR Primer 1 (100% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2820.22310832621285484No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA2750.217570176271401Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA2720.21519668343934933Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT2220.1756384695718219No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2110.16693566252096584Clontech SMART CDS Primer II A (100% over 26bp)
TATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT2070.16377100541156367Illumina Single End PCR Primer 1 (100% over 31bp)
AACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.15902401974746036No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG1950.15427703408335708Clontech SMART CDS Primer II A (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1790.1416184056457483Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1740.13766258425899552Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1710.1352890914269439Clontech Universal Primer Mix Long (96% over 26bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.1337067628722428No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAC1610.12737744865343842Illumina Single End PCR Primer 1 (96% over 33bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA1430.1131364916611285Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1430.1131364916611285Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGAG1400.11076299882907686Illumina Single End PCR Primer 1 (96% over 32bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA1350.10680717744232414Clontech SMART CDS Primer II A (96% over 26bp)
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.10047786322351973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)