FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001603021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001603021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962242
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA212342.206721386096221Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT211322.196121142082761Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG197262.050004053034476Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC158321.645324149226494Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC119151.2382539943174378Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC66950.695770918334473TruSeq Adapter, Index 5 (97% over 35bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG63570.6606446195447715Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG60120.624790853028656TruSeq Adapter, Index 6 (97% over 36bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA50400.523776763018035Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA49040.5096431043334213Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT40110.4168390072351862Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG37360.3882599179832101Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG36890.383375491820145Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC35230.3661241143080431Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA34990.36362993924605247Clontech SMART CDS Primer II A (96% over 26bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC33250.3455471700466203Clontech SMART CDS Primer II A (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT33040.3433647668673785Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCG31850.33099781551834156TruSeq Adapter, Index 6 (97% over 36bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC31550.3278800966908533Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT30740.31946225585663485Clontech SMART CDS Primer II A (96% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC30490.3168641568337279Clontech Universal Primer Mix Long (96% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC28610.29732645218146786Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA27850.2894282311518308Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC27090.2815300101221938Clontech Universal Primer Mix Long (96% over 25bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG25240.2623040773526826Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA24540.25502940008854325Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG23370.24287029666133883Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG23200.24110358932576212Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC23140.24048004556026445Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG22350.2322700526478786Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC21910.22769739836756242Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG20870.21688930643226964Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG20850.21668145851043707Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT20110.20899108540263261Clontech Universal Primer Mix Long (96% over 25bp)
ACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG19670.20441843112231642Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC19520.20285957170857227Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC18790.19527312256168405Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCT18430.19153185996869812Clontech SMART CDS Primer II A (96% over 26bp)
TTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGAT18080.18789452133662843Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT17870.18571211815738659Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG17420.18103553991615415Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA16910.17573541790942404Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC16660.17313731888651712Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC16400.1704352959026939Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC15690.16305669467763828Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA15650.16264099883397315Clontech SMART CDS Primer II A (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT15120.1571330289054105Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA14990.1557820174134989Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG14970.15557416949166633Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCC14680.1525603746250943TruSeq Adapter, Index 6 (97% over 36bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT14660.15235252670326174Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCC13750.14289544625988057TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC13620.141544434767969Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA13460.13988165139330855Clontech Universal Primer Mix Long (96% over 25bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC13100.1361403888003226Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA12820.13323051789466683Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG12780.13281482205100173Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA12740.1323991262073366Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC11040.11473205285156957Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10650.11067901837583476Clontech Universal Primer Mix Long (96% over 26bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGC10620.11036724649308594TruSeq Adapter, Index 6 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGATCAAGTGAATAAGTGCA10120.1051710484472721Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCAC10060.10454750468177444Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT9870.10257294942436518Clontech SMART CDS Primer II A (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)