FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001603025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001603025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919840
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG365113.969277265611411Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA349413.7985954078970257Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT275552.995629674726039Clontech SMART CDS Primer II A (100% over 26bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC245012.6636154113758916Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC183851.998717168203166Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG126731.3777396068881544Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA113801.2371716820316576Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA109501.1904244216385458Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC109291.1881414158984172TruSeq Adapter, Index 5 (97% over 35bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG108931.184227691772482TruSeq Adapter, Index 6 (97% over 36bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG88770.9650591407201252Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC84220.9155940163506697Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT83320.9058097060358323Clontech SMART CDS Primer II A (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA71640.7788311010610541Clontech SMART CDS Primer II A (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC68440.7440424421638545Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC67750.736541137589146Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT63730.6928378848495391Clontech SMART CDS Primer II A (96% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC61370.6671812489128545Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC61300.6664202469994782Clontech Universal Primer Mix Long (96% over 25bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT60220.6546790746216734Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA59890.6510914941728997Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA58100.6316315881022786Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC57980.6303270133936336Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCG56850.6180422682205601TruSeq Adapter, Index 6 (97% over 36bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC52800.574012871803792Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG52160.5670551400243521Clontech Universal Primer Mix Long (96% over 26bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG47530.5167202991824665Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG44340.48204035484432073Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC39750.4321403722386502Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG38870.4225734910419204Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG37440.4070273090972343Clontech SMART CDS Primer II A (96% over 26bp)
ACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG36730.39930857540441816Clontech SMART CDS Primer II A (96% over 26bp)
ACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCT36560.39746042790050445Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG36560.39746042790050445Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC35540.3863715428770221Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC35530.3862628283179684Clontech Universal Primer Mix Long (96% over 26bp)
TTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGAT34480.3748477996173248Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT34420.3741955122630023Clontech Universal Primer Mix Long (96% over 25bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT34110.3708253609323361Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA31150.3386458514524265Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC30940.3363628457122978Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC30410.3306009740824491Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30050.3266872499565142Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29940.32549138980692294Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA29180.31722908331883803Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA29140.31679422508262306Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA28510.3099452078622369Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA28430.30907549138980694Clontech Universal Primer Mix Long (96% over 25bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG26920.2926595929726909Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG26700.29026787267350845Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCC25100.27287354322490864TruSeq Adapter, Index 6 (97% over 36bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCC24920.27091668116194123TruSeq Adapter, Index 6 (97% over 36bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA24140.2624369455557488Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG22540.24504261610714906Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT21770.23667159506001045Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC21210.2305835797530005Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG21090.22927900504435555Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG20340.22112541311532444Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC20270.22036441120194816Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT19530.21231953383197077Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG19140.20807966602887457Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG19060.2072099495564446Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC18550.2016655070447034Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA18470.20079579057227345Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG18420.2002522177770047Clontech Universal Primer Mix Long (95% over 24bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA18310.19905635762741347Clontech Universal Primer Mix Long (96% over 26bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGC18000.19568620629674727TruSeq Adapter, Index 6 (97% over 36bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT17650.1918811967298661Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT16720.18177074273786745Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC16690.1814445990607062Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA16440.1787267350843625Clontech Universal Primer Mix Long (96% over 26bp)
CGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT16280.17698730213950253Clontech Universal Primer Mix Long (96% over 25bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGC16080.17481301095842755TruSeq Adapter, Index 6 (97% over 36bp)
CGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT16060.17459558184032004Clontech SMART CDS Primer II A (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCAC15690.17057314315533134Clontech SMART CDS Primer II A (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA14690.1597016872499565Clontech Universal Primer Mix Long (96% over 26bp)
AGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT14570.15839711254131153Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG14440.1569838232736128Clontech SMART CDS Primer II A (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA13730.14926508958079665Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC13240.14393807618716298Clontech Universal Primer Mix Long (96% over 26bp)
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG13220.1437206470690555Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA12710.1381762045573143Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG12510.13600191337623935Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT12410.13491476778570186Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC12390.13469733866759437Clontech SMART CDS Primer II A (96% over 26bp)
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11980.13024004174639067Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATCAACGCAGA11920.12958775439206818Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG11520.12523917202991824Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT11370.12360845364411202Clontech Universal Primer Mix Long (96% over 26bp)
CAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG11350.12339102452600453Clontech Universal Primer Mix Long (96% over 25bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG11210.12186902069925205Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11170.12143416246303705Clontech Universal Primer Mix Long (96% over 26bp)
TGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT11070.12034701687249957Clontech SMART CDS Primer II A (96% over 26bp)
CACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC10970.11925987128196208Clontech SMART CDS Primer II A (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG10350.11251956862062967Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA10210.1109975647938772Clontech Universal Primer Mix Long (96% over 25bp)
AACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCA10050.10925813184901723Clontech Universal Primer Mix Long (96% over 25bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT9960.10827970081753349Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9690.10534440772308228Clontech SMART CDS Primer II A (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA9570.10403983301443728Clontech SMART CDS Primer II A (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA9530.1036049747782223Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT9490.10317011654200732Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA9480.10306140198295356Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA9270.10077839624282484Clontech Universal Primer Mix Long (96% over 25bp)
ATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT9210.10012610888850235Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)