FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001603032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001603032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134147
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG252882.229693328995271Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA223691.9723192848898774Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG161171.4210679920680476Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG139421.2292939098723534Illumina Single End PCR Primer 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT98180.8656726156309543Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG93780.8268769392327449Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTC68920.6076813675828618Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAG64190.5659760154547867Illumina Single End PCR Primer 1 (96% over 33bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG49000.43204275988915014Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA43310.3818728965469203Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA40130.35383420315003256Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTA34520.3043697157423156Illumina Single End PCR Primer 1 (96% over 33bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT34010.2998729441597959Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG33800.2980213323316995Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC32250.28435467360051214Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG29500.26010737585163124Illumina Single End PCR Primer 1 (100% over 30bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC28840.25428802439189985Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC28080.24758695301402728Clontech Universal Primer Mix Long (96% over 25bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT27390.24150308557885353Clontech SMART CDS Primer II A (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA27130.2392106137916866Clontech SMART CDS Primer II A (96% over 26bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC25140.2216643874206783Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT23920.21090740441935657Clontech SMART CDS Primer II A (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC23430.20658697682046506Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA23290.2053525689350675Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG22980.20261923718883001Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC22510.19847515357356676Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21380.18851171849857204Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC19920.17563860769371165Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC19910.17555043570189754Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC19580.17264075997203185Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG19220.1694665682667238Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG19080.16823216038132624Clontech Universal Primer Mix Long (96% over 26bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG18660.16452893672513352Clontech Universal Primer Mix Long (96% over 25bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCA17920.1580042093308892Illumina Single End PCR Primer 1 (96% over 33bp)
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG17910.15791603733907508Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG17740.15641711347823517Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG17230.15192034189571546Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCA15490.13657841532005993Illumina Single End PCR Primer 1 (96% over 33bp)
ACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG15430.13604938336917524Clontech SMART CDS Primer II A (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG15160.1336687395901942Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC14690.12952465597493093Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC14320.12626229227780877Clontech SMART CDS Primer II A (96% over 26bp)
ACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCT14020.12361713252338542Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT13820.12185369268710317Clontech Universal Primer Mix Long (96% over 25bp)
TTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGAT13630.12017842484263505Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC13540.11938487691630802Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGT13130.11576982525192943Illumina Single End PCR Primer 1 (96% over 33bp)
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA12710.11206660159573671Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC12610.11118488167759558Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT12140.1070407980623323Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA12080.10651176611144762Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11840.10439563830790895Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA11700.10316123042251138Clontech SMART CDS Primer II A (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)