FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001603042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001603042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893790
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA457595.119658980297386Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG369714.13643025766679Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG100711.126774745745645Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA91081.0190313160809585Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT69230.7745667326776984Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA67330.7533089428165453Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG61830.6917732353237338Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC59930.6705154454625807Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC53780.6017073361751643Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA53310.5964488302621421Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC50830.5687018203381108Clontech Universal Primer Mix Long (96% over 25bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC50560.5656809765157363Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA50100.5605343537072467Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT48190.5391646807415612Clontech SMART CDS Primer II A (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG47920.5361438369191869Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC44500.49787981516911134Illumina Single End PCR Primer 1 (96% over 33bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA40570.4539097550878841Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG40540.4535741057742871Clontech Universal Primer Mix Long (96% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC39730.4445115743071638Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT39320.4399243670213361Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG39190.4384698866624151Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCG37590.4205685899372336Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG37340.41777151232392395Illumina Single End PCR Primer 1 (100% over 32bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC36280.40591190324349125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC33880.3790599581557189Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG32440.3629487911030555Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG31330.3505297664999608Clontech SMART CDS Primer II A (96% over 26bp)
ACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG31130.34829210440931313Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT30250.3384463912104633Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC29570.33083834010226115Clontech SMART CDS Primer II A (96% over 26bp)
ACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCT28550.3194262634399579Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG28530.31920249723089317Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTT28430.3180836661855693Illumina Single End PCR Primer 1 (96% over 33bp)
TTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGAT27380.306335940209669Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC27340.3058884077915394Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT26190.2930218507703152Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA25970.2905604224706027Clontech SMART CDS Primer II A (96% over 26bp)
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA25760.28821087727542266Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC25450.2847425010349187Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC24690.2762393850904575Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA24390.27288289195448595Clontech Universal Primer Mix Long (96% over 25bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG24180.27053334675930585Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC22920.25643607558822545Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA21220.23741594781772005Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG20730.23193367569563322Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC20220.22622763736448157Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT19620.2195146510925385Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC19290.2158225086429698Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA19220.2150393269112431Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG18780.2101164703118182Clontech Universal Primer Mix Long (96% over 25bp)
AGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC17680.1978093288132559Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA17620.1971380301860616Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCC17300.1935577708410253Illumina Single End PCR Primer 1 (96% over 33bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17030.1905369270186509Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG17010.19031316080958613Clontech Universal Primer Mix Long (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG16380.1832645252240459Clontech Universal Primer Mix Long (95% over 24bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG16320.1825932265968516Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTATCAACGCAGA15790.17666342205663524Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT15750.17621588963850568Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG14180.1586502422269213Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT13870.1551818659864174Clontech Universal Primer Mix Long (96% over 25bp)
TGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCAC13390.14981147696886293Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT13130.14690251625102094Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT12950.144888620369438Clontech Universal Primer Mix Long (96% over 26bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA12890.1442173217422437Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT12840.14365790621958177Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG12400.13873504962015687Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11860.1326933619754081Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT11540.1291131026303718Clontech SMART CDS Primer II A (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11510.12877745331677462Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTTCCATGTACTCTGCGTTGATACCACTGCTTTCCA10460.11702972734087425Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC10020.11210687074144933Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC10020.11210687074144933Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA9930.11109992280065788Clontech Universal Primer Mix Long (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA9910.11087615659159311Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT9790.10953355933720449Clontech SMART CDS Primer II A (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG9760.10919791002360735Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTC9510.10640083241029773Illumina Single End PCR Primer 1 (96% over 33bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC9400.10517011826044148Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGTACATGGA9250.10349187169245572Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA9210.10304433927432617Clontech Universal Primer Mix Long (96% over 26bp)
TCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT8940.10002349545195179Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)