FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001603043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001603043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433877
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC525473.664679745891733Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCG426712.975917739108724TruSeq Adapter, Index 1 (97% over 36bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG382352.6665467121656876TruSeq Adapter, Index 2 (97% over 35bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT382282.6660585252430997Clontech SMART CDS Primer II A (100% over 26bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC372002.5943647886115753Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA354412.471690389064055Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG302072.106666052945964Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGAT169981.1854573300220312TruSeq Adapter, Index 1 (97% over 36bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG134250.9362727765352259Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA112750.7863296503117073Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGC106790.7447640209027692TruSeq Adapter, Index 1 (97% over 36bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA99950.6970611844670079Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGC97810.6821366128335973TruSeq Adapter, Index 1 (97% over 36bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT88470.6169985291625433Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG79040.5512327765910185Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA74250.5178268428881975Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC72810.5077841404806689Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC69630.48560650599737637Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT68740.4793995579816121Clontech SMART CDS Primer II A (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC68560.478144220180671Clontech Universal Primer Mix Long (96% over 25bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC67210.46872918667361285Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA63920.44578440131196745Clontech Universal Primer Mix Long (96% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC58950.4111231298082053Clontech Universal Primer Mix Long (96% over 26bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGA58910.4108441658524406TruSeq Adapter, Index 1 (97% over 36bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT58540.4082637492616173Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC55480.3869230066456188Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA53700.3745091106140903Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGA52940.3692087954545613TruSeq Adapter, Index 1 (97% over 36bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC51580.359724020958562Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG50100.34940235459526864Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG49990.34863520371691575Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG49140.3427072196569162Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG48300.3368489765858578Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA42820.29863091464609587Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA42270.29479516025433145Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT42210.2943767143206844Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG41000.2859380546588027Clontech SMART CDS Primer II A (96% over 26bp)
ACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCT40490.2823812642228029Clontech SMART CDS Primer II A (96% over 26bp)
ACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG39800.2775691359858621Clontech SMART CDS Primer II A (96% over 26bp)
TTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGAT39120.27282674873786245Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC37950.26466705303174537Clontech SMART CDS Primer II A (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA37180.2592969968832752Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG36950.25769295413762827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC36290.25309004886751096Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC35060.24451190722774688Clontech SMART CDS Primer II A (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC34400.23990900195762954Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC34310.239281333057159Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG34070.23760754932257092Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG33740.23530609668751226Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA32390.22589106318045413Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA31540.21996307912045457Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30940.21577861978398427Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA30470.2125007933037492Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT29880.20838607495622008Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC28950.20190016298469116Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA28340.1976459626592797Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28200.1966695888141033Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG27090.18892833904163328Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG25870.18041993839081036Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA24550.17121412785057574Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC23890.16661122258045843Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA22800.15900945478587075Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT22710.15838178588540022Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG21850.15238406083645947Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21540.15022209017928317Clontech Universal Primer Mix Long (96% over 26bp)
AGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC21210.1479206375442245Clontech Universal Primer Mix Long (96% over 25bp)
TATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA20340.1418531715063426Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT19980.13934249590446043Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCAC19710.1374594892030488Clontech SMART CDS Primer II A (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG19360.1350185545901078Clontech Universal Primer Mix Long (95% over 24bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT18360.12804445569599066Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG17970.125324557127285Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG17590.12267439954752046Clontech SMART CDS Primer II A (96% over 26bp)
CGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT16880.11772278933269731Clontech Universal Primer Mix Long (96% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA16760.11688589746540325Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT16740.1167464154875209Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT16390.1143054808745799Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG16240.11325936604046233Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA16130.11249221516210943Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAG15980.11144610032799186TruSeq Adapter, Index 1 (97% over 36bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA15760.1099117985712861Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC15160.10572733923481581Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA14990.1045417424228159Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC14820.10335614561081598Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA14760.10293769967716895Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT14660.10224028978775726Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)