FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001603045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001603045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604609
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC541503.3746538876449033Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCG373562.3280437788894366TruSeq Adapter, Index 1 (97% over 36bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC366472.283858559935785Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG363362.2644768912551285TruSeq Adapter, Index 2 (97% over 35bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT234491.4613528903302924Clontech SMART CDS Primer II A (100% over 26bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGAT149190.9297592123688698TruSeq Adapter, Index 1 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA124550.7762015543973642Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG109550.682720837288087Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGC91560.5706062972350273TruSeq Adapter, Index 1 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT84320.5254862711102829Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGC83640.5212484786013291TruSeq Adapter, Index 1 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG83510.520438312386382Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG51170.31889388629878057Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGA48890.30468481729817043TruSeq Adapter, Index 1 (97% over 36bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGA44930.2800059079813213TruSeq Adapter, Index 1 (97% over 36bp)
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG44890.2797566260690299Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA39210.2443585945236503Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA38190.23800190576021948Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA37580.23420035659777552Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA31670.19736895405672036Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT31550.19662110831984614Clontech SMART CDS Primer II A (96% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC28870.17991922019632195Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC26710.16645799693258606Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC26410.1645883825904005Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG25950.16172164059904937Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA25300.15767080952431403Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT24410.15212428697583025Clontech SMART CDS Primer II A (96% over 25bp)
CTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTC24320.15156340267317459Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC24100.15019235215557186Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC23330.1453936753439623Clontech Universal Primer Mix Long (96% over 25bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT21790.13579632172074318Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA21630.13479919407157756Clontech Universal Primer Mix Long (96% over 25bp)
ATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA19360.12065244554904031Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA17950.11186525814076825Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG17730.11049420762316552Clontech Universal Primer Mix Long (96% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC17670.1101202847547284Clontech Universal Primer Mix Long (96% over 26bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT17120.10669265846072158Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC16810.10476072364046318Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG16770.10451144172817178Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16620.10357663455707901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG16610.10351431407900616Clontech SMART CDS Primer II A (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)