FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001603188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001603188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG190.7893643539675945Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA110.4570004154549232Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAG80.3323639385126714No Hit
CGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGT60.24927295388450355No Hit
CTCGGGCTCGCCCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGT60.24927295388450355No Hit
CTCGCTCACACTTTCTATATATTCAAAAGTGGGCAACTAGAGATTAATCT50.2077274615704196No Hit
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC50.2077274615704196No Hit
CTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGC40.1661819692563357No Hit
CTCCCCGGGGGCGGCCGCGACGCCCGCCGCAGCTGGGGCGATCCACGGGA40.1661819692563357No Hit
CTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGG40.1661819692563357No Hit
CTGCGCTAGTGAAAAATTCCACCAGCTAATGATCATCGTTTACGGCGTGG30.12463647694225177No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.12463647694225177Clontech Universal Primer Mix Long (96% over 26bp)
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA30.12463647694225177No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30.12463647694225177Clontech Universal Primer Mix Long (96% over 26bp)
CGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCAT30.12463647694225177No Hit
CGGCATTCTCACTTCTAAGCACTCCACTAATCCTCACGGTTTAGCTTCGC30.12463647694225177No Hit
CTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATG30.12463647694225177No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC30.12463647694225177No Hit
CGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGG30.12463647694225177No Hit
CGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT30.12463647694225177Clontech SMART CDS Primer II A (96% over 26bp)
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA30.12463647694225177No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT30.12463647694225177Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA30.12463647694225177No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA30.12463647694225177No Hit
CCACTTTTGAATATATAGAAAGTGTGAGCGAGACACCTTGTGAATTGAAA30.12463647694225177No Hit
GGAGAAGCGGAGACGCTAGTTTCTGTGGAATCAACCTTGAAATACCACCC30.12463647694225177No Hit
GCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC30.12463647694225177No Hit
CTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGGCAGCG30.12463647694225177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)