FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001603331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001603331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514743
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT290441.9174209750432911Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT251781.6621961613290177Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG235151.552408560396054Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA81630.5389032991075053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC68350.45123166108046053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC66770.44080084872483316Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC61880.40851814466216385Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG60160.3971630831104683Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAA25270.16682697989031803No Hit
GTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAG24130.15930095072233377No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC19320.12754638905741766TruSeq Adapter, Index 5 (97% over 35bp)
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG19170.12655612206163025No Hit
ATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCT19020.12556585506584286No Hit
ATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGA18150.11982230649027592No Hit
GTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG17270.11401274011498981Clontech SMART CDS Primer II A (96% over 26bp)
ATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATA17200.11355061551695568No Hit
ATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGA17090.11282441972004492No Hit
ATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCC16730.11044777893015514No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16670.1100516721318402No Hit
GTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCT16570.10939149413464859No Hit
GTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAG16270.10741096014307379No Hit
ATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGT16070.10609060414869056No Hit
CTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCA16060.1060245863489714No Hit
ATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTT16030.10582653294981394No Hit
ATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGG15750.10397803455767743No Hit
GTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGT15690.10358192775936248No Hit
ATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCG15560.1027236963630134No Hit
GTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCC15470.10212953616554096No Hit
GTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGG15410.10173342936722599No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT15400.10166741156750682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)