FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001603403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001603403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221224
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA189271.549838522662509Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT156811.284039619267227Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA156041.2777344696796Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC144671.1846311569376298Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA82380.6745691208164922Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT74660.6113538548210647Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG64660.5294687952414955Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG60930.49892566801831606Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG60330.4940125644435419Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG59960.4909828172390978Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC54160.44348948268294763Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC49900.40860644730205103Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC48560.39763384931838874Clontech SMART CDS Primer II A (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC48060.39353959633941027Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT42780.35030428488139764Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG41450.339413571957315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA38920.3186966518836839Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA38260.31329223795143235Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC37110.3038754560997819Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG29530.2418065809384683Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG27930.2287049714057372Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG27040.22141720110315555Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA26440.21650409752838137No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC26200.21453885609847168Clontech Universal Primer Mix Long (96% over 26bp)
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG24810.20315683281691158No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC23010.18841752209258908Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT22480.18407761393487188Clontech Universal Primer Mix Long (100% over 23bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA22380.1832587633390762Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC22340.18293122310075793Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA22210.18186671732622353Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT21090.17269559065331175Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC19020.1557453833203409Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA18730.15337071659253337Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG18630.1525518659967377No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA17310.14174303813223454Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA17240.14116984271517757Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG17120.14018722200022274Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC16630.13617485408082383No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16600.13592919890208513No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA16530.13535600348502813No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC16520.13527411842544856Clontech Universal Primer Mix Long (96% over 26bp)
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCG16150.1322443712210045No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC15970.13077044014857225Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG15520.12708561246749164Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT15120.12381021008430884Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT15060.12331889972683144Clontech Universal Primer Mix Long (96% over 26bp)
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14460.11840579615205729No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC14370.11766883061584116Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG14200.11627678460298847Clontech Universal Primer Mix Long (96% over 26bp)
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14050.11504850870929494No Hit
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA14040.11496662364971535Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13890.11373834775602182No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT13730.11242818680274871No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA13600.11136368102821431Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA13580.11119991090905518Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG13050.10686000275133802Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA12930.10587738203638317Clontech Universal Primer Mix Long (96% over 25bp)
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC12780.10464910614268962No Hit
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC12760.1044853360235305Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG12710.10407591072563264No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG12700.10399402566605308Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG12670.10374837048731436Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG12600.10317517507025739Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA12400.101537473878666No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT12370.10129181869992729No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)