FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001603774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001603774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462853
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG53840.36804791732320336No Hit
GTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTT40080.2739851509345095No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTT38610.2639362943508336No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT37000.25293040380680765No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT36630.2504010997687396No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT35970.2458893682413749No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG32450.22182680009542993No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG31240.21355529229526138No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT28540.19509820877422407No Hit
ATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAA27500.1879888136401949No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC26560.18156301419213003No Hit
CTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTG25190.1721977532944185No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA25170.17206103415722565No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA24090.16467820074881073No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA23890.16331100937688203No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA23400.15996139051565672No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA23160.1583207608693423No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA22880.15640669294864215No Hit
ACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTA22160.15148480400969885No Hit
TTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTG21500.14697307248233418No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT20880.14273477922935524No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC20780.14205118354339089No Hit
GTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTCCAC20350.1391117220937442No Hit
ATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCG20210.13815468813339413No Hit
TAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAG19810.13542030538953675No Hit
CTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGC19340.13220740566550432No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT18630.1273538762951575No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACC18370.1255765275116502No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT18010.12311558304217854No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAGACA17830.12188511080744271No Hit
ACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGG17650.12065463857270689No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT17510.1196976046123568No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA17430.11915072806358534No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA17410.11901400892639247No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT16540.11306672645850267No Hit
TTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTC16380.11197297336095972No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG16190.11067414155762746No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT15810.10807647795096295No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT15780.10787139924517365No Hit
GGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG15640.10691436528482356No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC15470.10575225261868416No Hit
GTACATGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC15260.10431670167815904No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG15190.103838184697984No Hit
CCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAG14800.10117216152272307No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA14760.10089872324833733No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)