FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001603968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001603968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1739727
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA65140.3744265623284573Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA62740.3606312944502212Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC59200.34028327432982297Clontech Universal Primer Mix Long (96% over 25bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC47360.2722266194638584No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT45130.2594085163936641Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT37490.21549358031461258No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA31560.1814077725988043Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT26860.15439203967059198Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG25790.14824164940821175Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG25010.14375818734778503Clontech Universal Primer Mix Long (96% over 26bp)
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA23750.13651567171171108No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT22910.13168732795432847No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC22450.1290432349443332No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA21480.1234676475102128No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21240.1220881207223892Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG20250.11639757272261682No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT19290.11087946557132239No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA18560.10668340492502561No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG18530.10651096407654763Clontech Universal Primer Mix Long (96% over 26bp)
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18100.10403931191503035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)