FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001604016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001604016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745262
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAGCCTGCCAAGG40680.23308821254344622No Hit
ATCCTAAGAGATAGGGTAATTCCGTTTTAATGTAGGCGCTTGCGCCTCGC40370.2313119749355684No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA38540.2208264432503544No Hit
ACTTATACCCCTCCGCTGGGGCAAAATTTACGCCCCAGCGAGTAGGCAGG37480.21475285659115936No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG31770.18203570581379758No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA30700.17590482116725167No Hit
CTTCTTGGGGCTCCCGCCAACGTCTCCGCTCTCAGTTGCGTTGCCGCGGA29520.16914365865984593No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGG28050.16072085451926416No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC27820.15940300081019354No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGG26570.1522407523913315No Hit
CTTCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTC26220.15023532283405014No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT26140.14977693893524296No Hit
TCTTAACAGTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAA26010.1490320650996813No Hit
CTTCCAGCCATTGAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACAG25790.1477715093779616No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTG25060.14358875630134615No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT24660.1412968368073103No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA23090.1323010527932196No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT22810.1306967091473945No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA22560.12926425946362208No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG22560.12926425946362208No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA22320.12788910776720055No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG22220.1273161278936916No Hit
GTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAAGCCGGTTAAA21950.1257690822352174No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTC21480.12307607682972528No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGG20740.11883602576575895No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG20610.1180911519301973No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTA20200.11574193444881056No Hit
GTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAACGTCTCCGCT20130.11534084853735428No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA20100.11516895457530159No Hit
GGGTAATTCCGTTTTAATGTAGGCGCTTGCGCCTCGCCCTCGAAAGGGAA19910.11408029281563456No Hit
GTCTGTCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCTGGA19840.11367920690417829No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAACGTACGCCGGACGGCCGAAG19050.10915266590345747No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG18660.10691804439677251No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGA18490.1059439786118073No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG18210.10433963496598217No Hit
GTCTAGATGAACCAACACCTTTTGTGGTGTCTGATGAGCGTATATTCCGG17920.1026779933328062No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA17650.10113094767433199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)