FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001604403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001604403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104350
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCAAACAACCCGACTCGCCGACAGCGCCTCGTGGTGCGACAGGGTCC57900.5242903065151447No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGA53380.4833612532258795No Hit
CCCTTAGTAACGGCGAGCGAACCGGGATAAGCCCACCATGAGAATCGGTC51070.4624439715669851No Hit
GTACATGGGGAGACCTCAGGTCAGGCGGGGCTACCCGCTGAATTTAAGCA49380.44714085208493687No Hit
TTCCCAATCCGTCCCCCGACCGGCACGCGGCGACCCGCTCTCGCCGCGGG48100.4355503237198352No Hit
TCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGA45740.4141802870466791No Hit
GAATTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCC42630.3860189251595961No Hit
GTATCAACGCAGAGTACATGGGGAGACCTCAGGTCAGGCGGGGCTACCCG38870.35197174808711007No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCCTTGGACGGAATT33530.30361751256395164No Hit
GGATAAGCCCACCATGAGAATCGGTCGCCTTCGGCGTTCGAATTGTAGTC30560.27672386471680177No Hit
TCTCCAGACTACAATTCGAACGCCGAAGGCGACCGATTCTCATGGTGGGC30400.27527504867116404No Hit
TAGTAACGGCGAGCGAACCGGGATAAGCCCACCATGAGAATCGGTCGCCT29710.2690270294743514No Hit
CTTCCAGGGGACTTGGGCCCGGTCCGCCGCTGAGGACGCTTCTCCAGACT29390.266129397383076No Hit
GTCCACCAACAGCCGACGGGTTCGGAACTGGGACCCCCGTGCCCAGCCCT29180.26422782632317654No Hit
TATCAACGCAGAGTACATGGGGAGACCTCAGGTCAGGCGGGGCTACCCGC27360.24774754380404762No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCCGCA27140.24575542174129578No Hit
CCATGAGAATCGGTCGCCTTCGGCGTTCGAATTGTAGTCTGGAGAAGCGT26730.24204283062434917No Hit
GCTTGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTGCCGGTCGGGGG26140.23670032145606013No Hit
CCCCTTGTCCGTACCAGTTCTGAGTTGACTGTTCGATGCCCGGGGAAGAG24690.22357042604246843No Hit
AACCGGGATAAGCCCACCATGAGAATCGGTCGCCTTCGGCGTTCGAATTG24660.22329877303391135No Hit
CGCCAATATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATT23870.21614524380857517No Hit
GAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGA23720.21478697876578984No Hit
GTGCGACAGGGTCCGAGCACAACGGGGCTCTCACCCTCTCCGGCGCCCCC23460.21243265269162856No Hit
GCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATC23090.2090822655860914No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAA23060.2088106125775343No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG22700.20555077647484943No Hit
GTTGGTGGACTGCTTGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTG22600.20464526644632589No Hit
GTTCTGAGTTGACTGTTCGATGCCCGGGGAAGAGGCCCCGAAGGGCCCGT22060.19975551229229863No Hit
GGTATCAACGCAGAGTACATGGGGAGACCTCAGGTCAGGCGGGGCTACCC21860.1979444922352515No Hit
TATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAA21830.19767283922669443No Hit
CTCCGGCGCCCCCTTCCAGGGGACTTGGGCCCGGTCCGCCGCTGAGGACG21830.19767283922669443No Hit
TTCCATGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAG21340.19323584008692898No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA21050.19060986100421062No Hit
GTACATGGGCAGAAATCACATTGCGTCAACATCCGCAGGGACCATCGCAA20480.18544845384162628No Hit
CAACGGGGCTCTCACCCTCTCCGGCGCCCCCTTCCAGGGGACTTGGGCCC20060.18164531172182732No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGA20050.18155476071897497No Hit
ATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCG19840.17965318965907548No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCCGCAG19280.1745823334993435No Hit
TAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGACTGT19260.17440123149363881No Hit
TTCCAGGGGACTTGGGCCCGGTCCGCCGCTGAGGACGCTTCTCCAGACTA19170.17358627246796757No Hit
ATATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAA19050.1724996604337393No Hit
GATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGACAGCGCCTCGTGG18570.16815321229682617No Hit
CTATCGGTCTCTCGCCAATATTTAGCCTTGGACGGAATTTACCGCCCGAT18400.16661384524833614No Hit
TTCCCAATCCGTCCCCCGACCGGCACGCGGCGAGAGCGGGTCGCCGCGTG18190.16471227418843665No Hit
GTACATGGGAGACCTCAGGTCAGGCGGGGCTACCCGCTGAATTTAAGCAT17950.16253905011998007No Hit
GCTCAAGCAGTCCACCAACAGCCGACGGGTTCGGAACTGGGACCCCCGTG17700.16027527504867117No Hit
CCTCAGGTCAGGCGGGGCTACCCGCTGAATTTAAGCATATCAATAAGCGG17430.15783039797165754No Hit
ACGCAGAGTACATGGGGAGACCTCAGGTCAGGCGGGGCTACCCGCTGAAT16330.14786978765789832No Hit
ATTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTT16200.14669262462081767No Hit
GCTCTGAGGGCTGGGCACGGGGGTCCCAGTTCCGAACCCGTCGGCTGTTG15990.1447910535609182No Hit
CTCTCACCCTCTCCGGCGCCCCCTTCCAGGGGACTTGGGCCCGGTCCGCC15740.14252727848960928No Hit
AAATCACATTGCGTCAACATCCGCAGGGACCATCGCAATGCTTTGTTTTA15660.1418028704667904No Hit
TTGGTGGACTGCTTGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTGC15590.14116901344682392No Hit
GTTCCGAACCCGTCGGCTGTTGGTGGACTGCTTGAGCTGCTCCCGCGGCG15520.14053515642685743No Hit
ATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGG15430.1397201974011862No Hit
ATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAAC15360.13908634038121973No Hit
GGTCCGAGCACAACGGGGCTCTCACCCTCTCCGGCGCCCCCTTCCAGGGG15120.13691311631276315No Hit
GATTGGGAACGGGCCCTTCGGGGCCTCTTCCCCGGGCATCGAACAGTCAA15110.1368225653099108No Hit
GTCCGAGCACAACGGGGCTCTCACCCTCTCCGGCGCCCCCTTCCAGGGGA15000.13582650427853488No Hit
CTTCTCCAGACTACAATTCGAACGCCGAAGGCGACCGATTCTCATGGTGG14970.1355548512699778No Hit
GAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAA14680.1329288721872595No Hit
CCAATATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCC14580.13202336215873592No Hit
GTCCGCCGCTGAGGACGCTTCTCCAGACTACAATTCGAACGCCGAAGGCG14570.13193281115588357No Hit
GTCCGTACCAGTTCTGAGTTGACTGTTCGATGCCCGGGGAAGAGGCCCCG14540.13166115814732648No Hit
GCTGAGGACGCTTCTCCAGACTACAATTCGAACGCCGAAGGCGACCGATT14390.13030289310454113No Hit
CCCTTCCAGGGGACTTGGGCCCGGTCCGCCGCTGAGGACGCTTCTCCAGA14180.12840132204464164No Hit
TTCTCATGGTGGGCTTATCCCGGTTCGCTCGCCGTTACTAAGGGAATCCT14150.12812966903608455No Hit
TCCCAGTTCCGAACCCGTCGGCTGTTGGTGGACTGCTTGAGCTGCTCCCG14060.12731471001041336No Hit
GGTCAGGCGGGGCTACCCGCTGAATTTAAGCATATCAATAAGCGGAGGAA13990.12668085299044687No Hit
GAGTACATGGGGAGACCTCAGGTCAGGCGGGGCTACCCGCTGAATTTAAG13920.12604699597048036No Hit
GTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTTGA13750.12450762892199031No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG13730.1243265269162856No Hit
TCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAG13560.12278715986779552No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCCGC13530.12251550685923847No Hit
GCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGA13430.12160999683071491No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCCGCAGGGACC13310.12052338479648662No Hit
GTCCTCAGCGGCGGACCGGGCCCAAGTCCCCTGGAAGGGGGCGCCGGAGA13230.11979897677366776No Hit
TACCAGTTCTGAGTTGACTGTTCGATGCCCGGGGAAGAGGCCCCGAAGGG13130.1188934667451442No Hit
CTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAAC13120.11880291574229183No Hit
GGGATAAGCCCACCATGAGAATCGGTCGCCTTCGGCGTTCGAATTGTAGT12820.11608638565672115No Hit
CTGGAGAAGCGTCCTCAGCGGCGGACCGGGCCCAAGTCCCCTGGAAGGGG12790.11581473264816408No Hit
CTACAATTCGAACGCCGAAGGCGACCGATTCTCATGGTGGGCTTATCCCG12650.11454701860823108No Hit
TATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGC12470.11291710055688867No Hit
GCTTGAGCTGCTCCCGCGGCGACCCGCTCTCGCCGCGGGAGCAGCTCAAG12460.11282654955403632No Hit
CCCCTGGAAGGGGGCGCCGGAGAGGGTGAGAGCCCCGTTGTGCTCGGACC12440.1126454475483316No Hit
GTCTGGAGAAGCGTCCTCAGCGGCGGACCGGGCCCAAGTCCCCTGGAAGG12400.11228324353692216No Hit
ATTCTCATGGTGGGCTTATCCCGGTTCGCTCGCCGTTACTAAGGGAATCC12240.11083442749128447No Hit
CCCTGGAAGGGGGCGCCGGAGAGGGTGAGAGCCCCGTTGTGCTCGGACCC12170.11020057047131795No Hit
GCTCTCACCCTCTCCGGCGCCCCCTTCCAGGGGACTTGGGCCCGGTCCGC12140.1099289174627609No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12100.10956671345135147Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCTACCCGCTGAATTTAAGCATATCAATAAGCGGAGGAAAAGAAACTA12000.10866120342282791No Hit
CACCCTCTCCGGCGCCCCCTTCCAGGGGACTTGGGCCCGGTCCGCCGCTG11970.10838955041427084No Hit
GAGCACAACGGGGCTCTCACCCTCTCCGGCGCCCCCTTCCAGGGGACTTG11720.10612577534296191No Hit
ATCCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTGTTAGTTTCTTTTCC11160.10105491918322995No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)