FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001605587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001605587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399302
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT46720.33388074911634513No Hit
ATCGTTAACTGAATACATAGGTTAACGAGGCGAACCGGGGGAACTGAAAC43290.3093685280232573No Hit
GGTTAACGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA33660.24054850203887368No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCTTCGAGTTCAC31130.22246805907516748No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC30060.21482138952134705No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29540.21110525104659322Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCAGACCGTTCCACTAACACACAAGCTGATTCAGACTCTGGGCTGCTC27610.19731266016914145No Hit
ACCCTGTACCGTCGGACTTTCCAGACCGTTCCACTAACACACAAGCTGAT27270.19488287732026396No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25240.18037564442843645Clontech Universal Primer Mix Long (96% over 26bp)
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA24580.1756590071335566No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC20420.14592989933552586No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC20160.14407183009814895No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT19990.1428569386737102No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC18300.1307794886307602No Hit
ATACATAGGTTAACGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC18290.1307080244293226No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCGTTAACCTATGTATTCAG17300.1236330684870028No Hit
GGATACCACGTGTCCCGCCCTACTCTTCGAGTTCACAGCCTGTGCATTTT17080.12206085605537619No Hit
GTTAACGATAGTGCAACGAATTGCACTGGGTTTCCCCATTCGGACATCGC16870.12056010782518713No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT16740.11963107320649867No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCTTCGAGTTCACAGCC16080.11491443591161879No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT15970.11412832969580548Clontech Universal Primer Mix Long (96% over 26bp)
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG15530.11098390483255223No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG15350.10969754920667589No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA15270.1091258355951753Clontech SMART CDS Primer II A (96% over 26bp)
GAATACATAGGTTAACGAGGCGAACCGGGGGAACTGAAACATCTAAGTAC15200.1086255861851123No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC15090.10783947996929899No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14920.10662458854486022No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAAA14680.10490944771035846No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG14530.10383748468879483No Hit
CTATCACCCTGTACCGTCGGACTTTCCAGACCGTTCCACTAACACACAAG14340.10247966486148094No Hit
GTACAGGGTGATAGTCCCGTACACCAAAATGCACAGGCTGTGAACTCGAA14000.10004988201260342No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)