FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001606003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001606003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2986190
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314391.0528131163790648No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194610.6516999922978779No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142230.4762925332949344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135000.4520810799044937No Hit
ATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGA130320.43640893580113793No Hit
TGCCTACATTGTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGATG116250.38929204102886955No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCG96390.3227858910518085No Hit
GTTTCATGCTCGGGGAAAGCCCCCGAAGGGGCGATTCCCGGTCCGTCCCC82880.2775442955739588No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGC78640.2633456009162176No Hit
GTACTCGGTCCTCCGGATTTTCATGGGCCGCCGGGGGCGCACCGGACACC74770.2503859432922888No Hit
GTACATGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAA74640.24995060595608454No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72270.2420140714422056No Hit
GTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGGCGATT69750.23357522461732172No Hit
TACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGG68090.22801630170886647No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66330.2221225039264079No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA66140.22148624166580155No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAA65540.2194769924217816No Hit
ATCCTAAGGGACGGGGTAACCCCGGCAGATAGCGCGATCACGCGCATCCC63740.21344924468972168No Hit
ACCGAGTACCGTTCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCA62750.21013398343708872No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGAA61390.20557968515064348No Hit
TGGGTAAGCCGATCCTAAGGGACGGGGTAACCCCGGCAGATAGCGCGATC61290.20524481027664015No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCGA60140.20139374922560185No Hit
TAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGT56760.19007497848428936No Hit
GCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGA56110.18789829180326773No Hit
GTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGA53930.18059801954999516No Hit
GTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGA51060.17098711066609962No Hit
GGCCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCG50260.16830811167407297No Hit
CCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTC48790.16338545102622407No Hit
GAGTACGACCGGGCGTGAACGGTACTCGGTCCTCCGGATTTTCATGGGCC45240.15149739299910586No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGC44640.1494881437550859No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACC41810.14001118482079172No Hit
GTGTCCGGTGCGCCCCCGGCGGCCCATGAAAATCCGGAGGACCGAGTACC41480.13890609773658075No Hit
GTCCGGTGCGCCCCCGGCGGCCCATGAAAATCCGGAGGACCGAGTACCGT41110.1376670607027684No Hit
TCTCCAAGGTGAACAGCCTCTGGCCAATGGAACAATGTAGGCAAGGGAAG41030.13739916080356573No Hit
GGGTAACCCCGGCAGATAGCGCGATCACGCGCATCCCCCGAAAGGGAATC40330.13505503668554245No Hit
GTTCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAG38920.1303333009620955No Hit
GTTATGAGTACGACCGGGCGTGAACGGTACTCGGTCCTCCGGATTTTCAT38700.1295965762392882No Hit
ACATTGTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTT38110.12762081448266857No Hit
AAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTA36950.12373626594422994No Hit
GCCTCTGGCCAATGGAACAATGTAGGCAAGGGAAGTCGGCAAAACGGATC36050.12072239207819999No Hit
TTCCCGGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGTG35990.12052146715379798No Hit
CTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGAAC35410.11857919288457867No Hit
GGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGGCGACGTTAGGAA35220.11794293062397235No Hit
GCTCTGAGGACTGGGCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTGTCG34360.11506300670754374No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG33590.1124844701777181No Hit
CCTCTGGCCAATGGAACAATGTAGGCAAGGGAAGTCGGCAAAACGGATCC33480.11211610781631444No Hit
GGGTAAGCCGATCCTAAGGGACGGGGTAACCCCGGCAGATAGCGCGATCA32990.11047522093369812No Hit
GAGTAGGAGGGCGCGGCGGCCGCTGCAAAACCCGGGGCGCGAGCCCGGGC32470.1087338715888808No Hit
GAACAATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAA32140.10762878450466984No Hit
TCCTAACGTCGCCGTCAACCGCCACATCCCGGCTCGGGAAATCTTAACCC31170.10438049822683754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)