FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001606004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001606004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2986190
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGA114680.3840345055070173No Hit
TGCCTACATTGTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGATG97610.3268713645146491No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCG84250.28213208134780443No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGC66600.22302666608621685No Hit
GTACATGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAA66120.22141926669100093No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAA63990.21428643187473No Hit
ACCGAGTACCGTTCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCA62140.20809124670566845No Hit
GTACTCGGTCCTCCGGATTTTCATGGGCCGCCGGGGGCGCACCGGACACC60890.20390531078062682No Hit
GTTTCATGCTCGGGGAAAGCCCCCGAAGGGGCGATTCCCGGTCCGTCCCC59460.19911660008237925No Hit
ACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGAA58050.1943948643589323No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA58020.19429440189673128No Hit
ATCCTAAGGGACGGGGTAACCCCGGCAGATAGCGCGATCACGCGCATCCC56130.18796526677806838No Hit
TACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGG55240.18498488039943875No Hit
GTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGGCGATT53990.18079894447439712No Hit
TGGGTAAGCCGATCCTAAGGGACGGGGTAACCCCGGCAGATAGCGCGATC53240.17828738291937218No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCGA52280.17507258412894022No Hit
GCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGA47740.1598692648491891No Hit
TAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGT47370.15863022781537678No Hit
GTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGA45380.15196621782271053No Hit
CCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTC43760.1465412448638566No Hit
GGCCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCG42730.14309203366162235No Hit
GTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGA39770.13317973739112382No Hit
GTGTCCGGTGCGCCCCCGGCGGCCCATGAAAATCCGGAGGACCGAGTACC39350.13177326292030983No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGC39250.1314383880463065No Hit
TCTCCAAGGTGAACAGCCTCTGGCCAATGGAACAATGTAGGCAAGGGAAG38450.12875938905427986No Hit
GAGTACGACCGGGCGTGAACGGTACTCGGTCCTCCGGATTTTCATGGGCC38220.1279891768440722No Hit
GTCCGGTGCGCCCCCGGCGGCCCATGAAAATCCGGAGGACCGAGTACCGT37380.12517622790244426No Hit
GTTCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAG37110.12427206574263526No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACC34680.11613460630435439No Hit
GGGTAACCCCGGCAGATAGCGCGATCACGCGCATCCCCCGAAAGGGAATC34080.11412535706033441No Hit
GCCTCTGGCCAATGGAACAATGTAGGCAAGGGAAGTCGGCAAAACGGATC33960.1137235072115304No Hit
CTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAATGGAAC33860.11338863233752708No Hit
AAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCTTTGTTTTA32570.10906874646288414No Hit
ACATTGTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTT31950.10699252224406351No Hit
GTTATGAGTACGACCGGGCGTGAACGGTACTCGGTCCTCCGGATTTTCAT31790.10645672244565818No Hit
GGTTAAGATTTCCCGAGCCGGGATGTGGCGGTTGACGGCGACGTTAGGAA31500.10548558531104851No Hit
GCTCTGAGGACTGGGCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTGTCG30870.10337587360482756No Hit
GACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCG30320.10153406179780926No Hit
CCTCTGGCCAATGGAACAATGTAGGCAAGGGAAGTCGGCAAAACGGATCC30060.10066338712540059No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)