FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001606043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001606043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2901116
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTATACGGCGCAAAGTGCGCCTTTGAAACGGAACTTCCCTATAGCTT194140.669190752799957No Hit
GTATAGCGAAAGGGAAGCCGGTCAATATTCCGGCACCTGGATGTGGGTTT185670.6399950915440816No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143180.49353421235138484No Hit
TATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTG108860.37523490960030553No Hit
ATCCTAAGCTATAGGGAAGTTCCGTTTCAAAGGCGCACTTTGCGCCGTAT92390.3184636532975586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87810.3026766251332246No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTCATGGCTGGA80020.2758248894563334No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATTGGTACGACCTG78170.2694480331017443No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGA74200.2557636440597343No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT69310.23890806158733396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65440.22556836748340983No Hit
GTGATAGACTGTTAAGAAGAAAAGGGAACTCTGCCCGGGGCCCCCGCCGT59530.20519689664253343No Hit
GTTCTAAGTTGGTTGTTAACTGTACGCCGGACGGCCGAAGCCTGCCAAGG58470.20154313029882293No Hit
GTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGG55530.19140909911909762No Hit
TCCCTATAGCTTAGGATCGACTAACCCACGTCCAACCGCTGTTCACATGG55100.18992691088532826No Hit
GCTTAGGATCGACTAACCCACGTCCAACCGCTGTTCACATGGAACCCTTC53580.18468754782642266No Hit
GTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATTGG51870.17879326438515386No Hit
CCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCGACTT49150.1694175620692175No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAACTGTACGCCGGACGGCCGAAG48010.16548803977503831No Hit
GCCTAGGGCGTGAGCCCGGGTTGAACGGCCTCTAGTGCAGATCTTGGTGG47180.16262707178892535No Hit
ATCCTTAGCGGATCCCGACTTCCATGGTCACCGTCCTACTGTCAAGATGT46480.1602142072223241No Hit
CTTCTTAACAGTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTT43970.15156236427636813No Hit
GTGTAACAACTCACCTACCGAATGTACTAGCCCTGAAAATGGATGGCGCT43650.15045934047449327No Hit
GCTATAGGGAAGTTCCGTTTCAAAGGCGCACTTTGCGCCGTATAGCGAAA42350.14597830627937664No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTT41690.14370331968800973No Hit
ATATTGCCTTCATCCATTCTCAAACTTTAAATATGTAAGAAGCTCTTGTT40910.14101469917093973No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCA40120.13829160916006114No Hit
GTATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGCAGCGGTTCTG39350.13563745813679975No Hit
GTTAAGAAGAAAAGGGAACTCTGCCCGGGGCCCCCGCCGTCGTCTCCGCG39200.13512041572967093No Hit
CACCCATACCTCGCCCTCAGGGTAGAAACCATGCCCTGAGGAGTAGGCGG38130.13143217989215183No Hit
ACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTT37660.12981211368314816No Hit
CCCTTATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCT37520.1293295407698279No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTC37520.1293295407698279No Hit
CCTTGGAGACCTGCTGCGGTTATTGGTACGACCTGGCGTGAGAATTATTC37470.12915719330078493No Hit
GCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC37230.1283299254493788No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA37040.1276750050670156No Hit
CTTCCAGCCATGAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAG36750.1266753897465665No Hit
CCCATACCTCGCCCTCAGGGTAGAAACCATGCCCTGAGGAGTAGGCGGAC36690.12646857278371496No Hit
GCTAAGGATTGTGTAACAACTCACCTACCGAATGTACTAGCCCTGAAAAT35830.12350419631617626No Hit
TCTCCAAGGTGAACAGCCTCTGGTTGATAGAACAATGTAGATAAGGGAAG35690.12302162340285601No Hit
CGCCAGGTCGTACCAATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCT35330.12178072162574678No Hit
GAATAATTCTCACGCCAGGTCGTACCAATAACCGCAGCAGGTCTCCAAGG35300.12167731314432101No Hit
CTATAGGGAAGTTCCGTTTCAAAGGCGCACTTTGCGCCGTATAGCGAAAG34890.12026406389816884No Hit
CCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACTGTACGCCGGACGG34870.12019512491055168No Hit
CTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTTCAATGAGAACTTGA34130.11764438236871605No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACT33720.11623113312256388No Hit
GGGTAGAAACCATGCCCTGAGGAGTAGGCGGACGTAGAGGTCGTGACGAA33220.1145076584321344No Hit
GTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACTGTACG32790.11302547019836505No Hit
CCCTAGGCTTCGTCACGACCTCTACGTCCGCCTACTCCTCAGGGCATGGT32710.11274971424789632No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGC32630.11247395829742761No Hit
GTACTAGCCCTGAAAATGGATGGCGCTCAAGCGTCTCACCCATACCTCGC32430.11178456842125582No Hit
TCCTTATCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACAT31950.11013003271844353No Hit
GACCTGGCGTGAGAATTATTCCCTCCCGCGGATTTTCAAGGGACGTCGAG31490.10854443600324841No Hit
CCCTATAGCTTAGGATCGACTAACCCACGTCCAACCGCTGTTCACATGGA31180.10747588169518214No Hit
TCTTAACAGTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTCA30980.10678649181901033No Hit
ACCCAGCAGAGGTGCTGGGCTCTTCCAGCCATGAAACCCTAGCTCCGGAC30920.10657967485615881No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30790.10613157143664713No Hit
GAATTATTCCCTCCCGCGGATTTTCAAGGGACGTCGAGGGCGCACCGGAC30750.10599369346141278No Hit
AGCCTAGGGCGTGAGCCCGGGTTGAACGGCCTCTAGTGCAGATCTTGGTG30580.10540771206666677No Hit
TAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGT30160.103959993326706No Hit
GCACTAGAGGCCGTTCAACCCGGGCTCACGCCCTAGGCTTCGTCACGACC29810.10275356104340536No Hit
CCTCTGCTGGGTCCGGTGCGCCCTCGACGTCCCTTGAAAATCCGCGGGAG29580.10196076268580781No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAA29160.10051304394584704No Hit
GCTAGTGGCGGCCTGCTCCCGAGGCGTCCTTCCAAGGCCCAACGTACCCA29080.10023728799537833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)