FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001607391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001607391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1672721
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCG52510.31391965545957756No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA41100.2457074431420422Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA40110.23978894268679596No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA38030.22735411344749065No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG37810.22603889112410258No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT34990.2091801322515829Clontech Universal Primer Mix Long (96% over 26bp)
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG33950.20296271763193025No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG33110.1979409596699031No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC32680.19537029785600826No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA32140.1921420248804194No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT30970.18514743343331017No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA30760.18389199394280337No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC29690.1774952308245069No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT29430.17594087716959372No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA28990.1733104325228176No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG24630.1472451173865815No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT24390.14581032939743088No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA24280.14515271823573686No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA24010.14353858174794243No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT23760.14204401092591054No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT23610.1411472684326914No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG23550.14078857143540374No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC23130.13827769245439017No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC23130.13827769245439017No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC22690.13564724780761406No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA22640.13534833364320767No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG22220.1328374546621941No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG22180.132598323330669No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG21970.1313428838401622No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC21610.1291907018564363No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21210.12679938854118528Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC21170.1265602572096602No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA20400.12195697907780197No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG20210.12082110525305774No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT19660.1175330494445876No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG19630.11735370094594377No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG19560.11693522111577484No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATG19160.11454390780052381No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA18820.11251129148256045No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC18230.1089841043425652No Hit
GGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGAGCCATTC18220.10892432150968391No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG18130.10838627601375245Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG17980.10748953352053331Clontech Universal Primer Mix Long (96% over 26bp)
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG17660.10557648286833249No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC17490.1045601747093508No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA17430.10420147771206317No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC17420.10414169487918189No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA16980.10151125023240576Clontech SMART CDS Primer II A (96% over 26bp)
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC16910.10109277040223684No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG16910.10109277040223684No Hit
TCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAA16880.10091342190359301No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT16790.10037537640766153No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)