FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001607899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001607899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586814
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAGCGTTCCACTAAAACACACATAACTTAATGGGCTGCTCCCCGT53310.33595619902521656No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42430.267391137209528Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTATAAGGCGGGTATCATAAACTGAATACATAGGTTTATGAAGCGAACGA39880.251321200846476No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35250.2221432379598365Clontech Universal Primer Mix Long (96% over 26bp)
ATCATAAACTGAATACATAGGTTTATGAAGCGAACGAGGGGAAGTGAAAC32690.20601028223849802No Hit
GGTTTATGAAGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAA26960.16990018994034586No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT26760.1686398027746163No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA23580.14859964683951618No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT23330.1470241628823542Clontech Universal Primer Mix Long (96% over 26bp)
TCATAAACCTATGTATTCAGTTTATGATACCCGCCTTATAGCGGGTGGGT23170.14601585314977056No Hit
GTATCAACGCAGAGTACATGGGGTCAAGTAATTAAGTGCATGTGGTGGAT21890.1379493752891013No Hit
TCATTATGTGTGCCCTTTCGTGTACGGGAATATCACCCTCTACGTTCGCA19950.1257236197815245No Hit
ACTTAATGGGCTGCTCCCCGTTCGCTCGCCGCTACTAAGGGAATCTCAAT19930.12559758106495153No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG18620.11734204512942287Clontech Universal Primer Mix Long (96% over 26bp)
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG17890.11274163197450993No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17370.10946462534361305No Hit
TCTCAGTACCCTTAGGAAAAGAAATCAATTGAGATTCCCTTAGTAGCGGC17290.10896047047732123No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA17290.10896047047732123No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG17150.10807819946131053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATATTCAGACAAGGTTTCACGTGCCTCGCCCTACTCGTCATCATTAT16700.10524232833841901No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA16320.1028475927235328Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)