FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001607900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001607900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586814
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAGCGTTCCACTAAAACACACATAACTTAATGGGCTGCTCCCCGT56580.35656352918489503No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42850.2700379502575601Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTATAAGGCGGGTATCATAAACTGAATACATAGGTTTATGAAGCGAACGA39470.24873740715673037No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36690.23121802555308937Clontech Universal Primer Mix Long (96% over 26bp)
ATCATAAACTGAATACATAGGTTTATGAAGCGAACGAGGGGAAGTGAAAC33000.20796388234537885No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT27680.17443758373697232No Hit
GGTTTATGAAGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAA27610.17399644822896695No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA24580.154901582668164No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT24280.15301100191956965Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGGTCAAGTAATTAAGTGCATGTGGTGGAT23550.14841058876465674No Hit
TCATAAACCTATGTATTCAGTTTATGATACCCGCCTTATAGCGGGTGGGT23550.14841058876465674No Hit
TCATTATGTGTGCCCTTTCGTGTACGGGAATATCACCCTCTACGTTCGCA20330.12811835539641067No Hit
ACTTAATGGGCTGCTCCCCGTTCGCTCGCCGCTACTAAGGGAATCTCAAT19920.12553456170666505No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG19890.1253455036318056No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18490.11652279347169864No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG18460.11633373539683921Clontech Universal Primer Mix Long (96% over 26bp)
TCTCAGTACCCTTAGGAAAAGAAATCAATTGAGATTCCCTTAGTAGCGGC18280.11519938694768259No Hit
CCCATATTCAGACAAGGTTTCACGTGCCTCGCCCTACTCGTCATCATTAT17210.10845631561102939No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA16750.10555742512985138Clontech SMART CDS Primer II A (96% over 26bp)
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA16570.10442307668069477No Hit
GTACATGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA16460.10372986373954352No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG16380.10322570887325169Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGT15990.10076795390007902No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)