FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001608268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001608268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1018327
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA413214.057733910620066Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT373783.670530193150137Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG193741.902532290708191Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT165351.6237416861185061Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG126831.245474194438525Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC125291.2303513507939985Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG124551.223084529821953Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC113761.1171264240268597Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT77920.7651766082996916Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC68260.6703151345294782Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG62510.613849971571018Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC52300.5135874822134736Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT50270.49365282468205207Clontech Universal Primer Mix Long (100% over 23bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA49960.4906086158964654Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG49430.4854040008759465Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC45050.44239227674411064Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC43950.43159024556944875Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC42210.4145033962568016Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG40460.39731834666074844Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC39550.3883821208708008Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA38650.3795440953642592Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA36690.3602968398166797Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG32790.32199872928833273Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT32460.3187581199359341Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG31600.3103128955630166Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG31370.3080542890446782Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT28800.2828168162093316Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC28350.2783978034560608Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA27260.2676939725648048Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG27050.2656317666132784Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC26650.2617037552770377Clontech Universal Primer Mix Long (96% over 25bp)
GCTTAACATGTACTCTGCGTTGATACCACTGCTTAACATGTACTCTGCGT21380.20995220592206632Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA21120.20739899855350985Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG20840.20464939061814133Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20650.202783585233427Clontech SMART CDS Primer II A (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20140.19777537077972007Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC17620.17302889936140356Clontech Universal Primer Mix Long (96% over 25bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA17580.17263609822777948Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA16070.15780785543347078Clontech Universal Primer Mix Long (96% over 26bp)
TACCTCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC15840.15554924891513236Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT15650.15368344353041802Clontech SMART CDS Primer II A (96% over 26bp)
ATGTTAAGCAGTGGTATCAACGCAGAGTACATGTTAAGCAGTGGTATCAA15010.1473986253924329Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGTTAAGCAGTGGTATCAACGCAGAGTACATGTTAAGCAGTGG14400.1414084081046658Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC12550.12324135567455248Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC12340.1211791497230261Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12180.11960794518852981No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC11820.11607273498591318Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11560.1135195276173567No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC10640.10448510154400306No Hit
CATGTACTCTGCGTTGATACCACTGCTTAACATGTACTCTGCGTTGATAC10540.10350309870994288Clontech SMART CDS Primer II A (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10350.10163729332522853Clontech Universal Primer Mix Long (96% over 26bp)
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10310.10124449219160447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)