FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001608373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001608373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1203287
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA74990.623209591726662Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA74700.6207995266299727Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC66620.553650126694629Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT57830.4806002225570458Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT38610.32087108063163655Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA36280.30150745416513264Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG34160.28388904725140385Clontech SMART CDS Primer II A (96% over 26bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC33600.27923512844400383No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG32260.2680989655834394Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG29800.2476549651080748Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG23350.1940517931299848Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC21610.17959140254984887Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT19970.16596206889960582No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG18350.15249894663534136Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT17620.1464322310471234Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC17460.1451025399592948Clontech SMART CDS Primer II A (96% over 26bp)
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC16770.13936824714303403No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA16770.13936824714303403No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA16670.13853719021314118No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC16510.13720749912531258Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC16210.134714328335634Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC15130.125738913492791Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15010.12474164517691955No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14880.12366127116805882Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA14660.12183294592229453Clontech SMART CDS Primer II A (96% over 26bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT14320.11900735236065876No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG14080.11701281572891588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)