FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001608875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001608875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448034
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA83131.8554395425347183Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT62611.3974385872500747Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG42200.9418928027783605Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT40760.9097523848636487Clontech Universal Primer Mix Long (96% over 26bp)
CTCGCCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCA26560.5928121526491293No Hit
GTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCA24040.5365664212983836No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG23460.523620975193847Clontech Universal Primer Mix Long (96% over 26bp)
CGCCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATT23120.5160322654084288No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG22140.49415892543869433Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21400.4776423217880786Clontech SMART CDS Primer II A (96% over 26bp)
TTCCCAAACAACCCGACTCGTTGACGGCGCCTCGTGGGGCGACAGGGTCC20790.4640272836436521No Hit
GTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAA19970.44572510121999676No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC19790.4417075489806578Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT16810.3751947396849346Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC15820.35309820236857026Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC15820.35309820236857026Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCG15630.34885745278260133No Hit
GTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACAAGGATTCCCCT13730.3064499569229121No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG13330.2975220630577144Clontech Universal Primer Mix Long (96% over 26bp)
GTTTCATGCTCGGGGAAAGCCCCCGAAGGGGCGATTCCCGGTCCGTCCCC13300.2968524710178245No Hit
ATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGA12900.2879245771526268No Hit
GCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAAACAACCC12110.2702919867688613No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGC11000.24551708129293756No Hit
CCCCTAGTAACGGCGAGCGAACCGGGAGCAGCCCAGCTTGAGAATCGGGC10970.24484748925304775No Hit
TATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCGC10900.24328510782663815No Hit
GAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACAAGGATTCCC10760.24016034497381894No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10310.2301164643754715Clontech Universal Primer Mix Long (100% over 23bp)
GAGTCTACCGCCCGATTTGGGCTGCATTCCCAAACAACCCGACTCGTTGA10030.2238669386698331No Hit
TATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAAA9860.22007258377712405No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG9760.21784061031082463Clontech Universal Primer Mix Long (96% over 26bp)
TAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGT9650.21538543949789524No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA9440.21069829521866645Clontech SMART CDS Primer II A (96% over 26bp)
GCATATAAATAAGCGGAGGAGAAGAAACTTACAAGGATTCCCCTAGTAAC9330.20824312440573706No Hit
GGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGGACTACCC9330.20824312440573706No Hit
GTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGGCGATT9300.20757353236584725No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC9250.20645754563269753Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC9050.20199359870009867Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAA8690.1939584942214207No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA8530.1903873366753416Clontech Universal Primer Mix Long (96% over 26bp)
GATTTGGGCTGCATTCCCAAACAACCCGACTCGTTGACGGCGCCTCGTGG8400.18748577116915233No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA8340.18614658708937268No Hit
TACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGG8330.18592338974274272No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC8060.17989706138373426Clontech Universal Primer Mix Long (96% over 26bp)
GTCCGGGCCGGACGGGGCTCTCACCCTCCCAGGCGCCCCTTTCCAGGGGA7890.17610270649102525No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA7710.17208515425168627Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGA7710.17208515425168627No Hit
GCTCTGAGGACTGGGCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTGTCG7560.1687371940522371No Hit
TAGTAACGGCGAGCGAACCGGGAGCAGCCCAGCTTGAGAATCGGGCGGCT7360.16427324711963825No Hit
GGTCCGGGCCGGACGGGGCTCTCACCCTCCCAGGCGCCCCTTTCCAGGGG7320.16338045773311846No Hit
GAGTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAG7250.16181807630670886No Hit
CGCATGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATT7070.15780052406736989No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG7060.15757732672073993Clontech Universal Primer Mix Long (96% over 26bp)
GTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGA7050.15735412937411No Hit
GCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGA6970.15556855060107044No Hit
GTACTTGTTCGCTATCGGTCTCTCGCATGTATTTAGCCTTGGACGGAGTC6880.15355977448140096No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA6880.15355977448140096Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG6650.14842623550891226Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TAAATAAGCGGAGGAGAAGAAACTTACAAGGATTCCCCTAGTAACGGCGA6620.14775664346902245No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG6460.14418548592294333Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6350.14173031511001397Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATAAATAAGCGGAGGAGAAGAAACTTACAAGGATTCCCCTAGTAACGG6350.14173031511001397No Hit
ACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCG6310.1408375257234942No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC6280.14016793368360436Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA6190.13815915756393488Clontech Universal Primer Mix Long (96% over 25bp)
TGCCTACATTGTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGATG6160.13748956552404507No Hit
CTTACAAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAGCAGCCCAGCT6120.13659677613752527No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT6020.13436480267122586Clontech Universal Primer Mix Long (96% over 26bp)
GGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTT6000.13391840797796598No Hit
TCCCAGAGCGTTCCACTAAAACACACATAACTTAATGGGCTGCTCCCCGT5880.13124003981840665No Hit
CCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTC5650.12610650084591793No Hit
GAACAATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAA5630.12566010615265805No Hit
GTCTACCGCCCGATTTGGGCTGCATTCCCAAACAACCCGACTCGTTGACG5570.12432092207287838No Hit
TTCCAGGGGACTTGGGCCCGGTCCGTCGCTGAGGACGCCTCTCCAGACTA5500.12275854064646878No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG5470.12208894860657896Clontech SMART CDS Primer II A (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5430.12119615922005919Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGT5400.12052656718016935No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC5380.12008017248690947Clontech Universal Primer Mix Long (96% over 26bp)
TCTCCAGACTACAATTCGGACGGCACAGCCGCCCGATTCTCAAGCTGGGC5190.11583942290094056No Hit
ATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAAAC5180.1156162255543106No Hit
GCTTGAGAATCGGGCGGCTGTGCCGTCCGAATTGTAGTCTGGAGAGGCGT5140.11472343616779083No Hit
GCATTCCCAAACAACCCGACTCGTTGACGGCGCCTCGTGGGGCGACAGGG5090.11360744943464111No Hit
CTATCGGTCTCTCGCATGTATTTAGCCTTGGACGGAGTCTACCGCCCGAT5010.11182187066160157No Hit
CATATAAATAAGCGGAGGAGAAGAAACTTACAAGGATTCCCCTAGTAACG4980.11115227862171174No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT4790.10691152903574283Clontech Universal Primer Mix Long (96% over 26bp)
TTTCCAGGGGACTTGGGCCCGGTCCGTCGCTGAGGACGCCTCTCCAGACT4720.10534914760933323No Hit
GTACATGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCAT4660.10400996352955356No Hit
GTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTCCCCTGGAAAG4660.10400996352955356No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGGAGTC4660.10400996352955356No Hit
CCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCC4600.1026707794497739No Hit
CATGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCC4540.10133159536999424No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)