FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001608876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001608876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448034
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA81351.8157104148345884Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT64541.4405156751496537Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG41100.9173410946490669Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT39900.8905574130534737Clontech Universal Primer Mix Long (96% over 26bp)
CTCGCCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCA27110.6050880067137763No Hit
GTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCA25130.5608949320810473No Hit
CGCCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATT24400.5446015257770616No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG23650.5278617247798159Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCAAACAACCCGACTCGTTGACGGCGCCTCGTGGGGCGACAGGGTCC22480.5017476352241124No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG22320.4981764776780333Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAA21890.48857899177294584No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21660.4834454528004571Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC19840.44282353571380745Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCG16250.3626956882736578No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT15340.34238472973033296Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC15110.33725119075784427Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC14790.3301088756656861Clontech Universal Primer Mix Long (96% over 26bp)
GTTTCATGCTCGGGGAAAGCCCCCGAAGGGGCGATTCCCGGTCCGTCCCC13670.3051107728431324No Hit
GCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAAACAACCC13570.302878799376833No Hit
GTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACAAGGATTCCCCT13260.29595968163130476No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG12770.28502301164643756Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGA12310.2747559337014602No Hit
TATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCGC11450.2555609618912851No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGC11400.2544449751581353No Hit
GAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACAAGGATTCCC10720.23926755558729917No Hit
GAGTCTACCGCCCGATTTGGGCTGCATTCCCAAACAACCCGACTCGTTGA10580.23614279273447997No Hit
TATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAAA10570.23591959538785No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10190.22743809621591218Clontech Universal Primer Mix Long (100% over 23bp)
TAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGT10070.22475972805635286No Hit
GTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGGCGATT10060.22453653070972293No Hit
GTACATGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAA9440.21069829521866645No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA9190.20511836155291788Clontech SMART CDS Primer II A (96% over 26bp)
CCCCTAGTAACGGCGAGCGAACCGGGAGCAGCCCAGCTTGAGAATCGGGC9180.20489516420628792No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC9050.20199359870009867Clontech Universal Primer Mix Long (96% over 25bp)
GCATATAAATAAGCGGAGGAGAAGAAACTTACAAGGATTCCCCTAGTAAC9030.2015472040068388No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG8780.1959672703410902Clontech Universal Primer Mix Long (96% over 26bp)
GATTTGGGCTGCATTCCCAAACAACCCGACTCGTTGACGGCGCCTCGTGG8660.19328890218153086No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC8550.1908337313686015Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGG8450.18860175790230205No Hit
GGTATCAACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGGACTACCC8360.18659298178263256No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA8230.1836914162764433Clontech Universal Primer Mix Long (96% over 26bp)
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA8060.17989706138373426No Hit
GTCCGGGCCGGACGGGGCTCTCACCCTCCCAGGCGCCCCTTTCCAGGGGA7700.17186195690505632No Hit
GTACTTGTTCGCTATCGGTCTCTCGCATGTATTTAGCCTTGGACGGAGTC7630.17029957547864671No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA7630.17029957547864671Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTCCGGGCCGGACGGGGCTCTCACCCTCCCAGGCGCCCCTTTCCAGGGG7630.17029957547864671No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC7610.16985318078538683Clontech Universal Primer Mix Long (96% over 26bp)
CGCATGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATT7460.1665052205859377No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA7380.16471964181289814Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAG7330.1636036550797484No Hit
GTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGA7300.16293406303985858No Hit
GTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGA7280.1624876683465987No Hit
GCTCTGAGGACTGGGCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTGTCG7170.1600324975336693No Hit
GCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGTTCTGA6800.15177419570836143No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG6760.15088140632184163Clontech Universal Primer Mix Long (96% over 26bp)
TAAATAAGCGGAGGAGAAGAAACTTACAAGGATTCCCCTAGTAACGGCGA6700.14954222224206198No Hit
GGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTT6580.14686385408250266No Hit
TAGTAACGGCGAGCGAACCGGGAGCAGCCCAGCTTGAGAATCGGGCGGCT6550.14619426204261285No Hit
ACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCG6480.14463188061620325No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC6330.14128392041675408Clontech Universal Primer Mix Long (96% over 26bp)
ATATAAATAAGCGGAGGAGAAGAAACTTACAAGGATTCCCCTAGTAACGG6250.13949834164371455No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG6240.1392751442970846Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG6240.1392751442970846Clontech Universal Primer Mix Long (96% over 26bp)
GTCTACCGCCCGATTTGGGCTGCATTCCCAAACAACCCGACTCGTTGACG6210.13860555225719476No Hit
TTCCAGGGGACTTGGGCCCGGTCCGTCGCTGAGGACGCCTCTCCAGACTA6140.13704317083078515No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT6100.1361503814442654Clontech Universal Primer Mix Long (96% over 26bp)
ATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAAAC6050.13503439471111567No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG6000.13391840797796598Clontech SMART CDS Primer II A (96% over 26bp)
TGCCTACATTGTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGATG5940.1325792238981863No Hit
CCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTC5870.1310168424717767No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5770.12878486900547728Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTACAAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAGCAGCCCAGCT5750.1283384743122174No Hit
GCATTCCCAAACAACCCGACTCGTTGACGGCGCCTCGTGGGGCGACAGGG5690.1269992902324377No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA5680.12677609288580777Clontech Universal Primer Mix Long (96% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC5650.12610650084591793Clontech Universal Primer Mix Long (96% over 25bp)
GAACAATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAA5590.12476731676613828No Hit
CATATAAATAAGCGGAGGAGAAGAAACTTACAAGGATTCCCCTAGTAACG5520.12320493533972868No Hit
ACGCAGAGTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGT5500.12275854064646878No Hit
TCCCAGAGCGTTCCACTAAAACACACATAACTTAATGGGCTGCTCCCCGT5460.12186575125994902No Hit
TCTCCAGACTACAATTCGGACGGCACAGCCGCCCGATTCTCAAGCTGGGC5430.12119615922005919No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGGAGTC5230.11673221228746032No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC5100.11383064678127106Clontech Universal Primer Mix Long (96% over 26bp)
CCTGTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCC4990.11137547596834169No Hit
CTATCGGTCTCTCGCATGTATTTAGCCTTGGACGGAGTCTACCGCCCGAT4880.10892030515541232No Hit
GCTTGAGAATCGGGCGGCTGTGCCGTCCGAATTGTAGTCTGGAGAGGCGT4770.10646513434248293No Hit
GTACATGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCAT4730.10557234495596317No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT4710.10512595026270329Clontech Universal Primer Mix Long (96% over 26bp)
CCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGG4510.10066200333010442No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)