FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001609488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001609488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817718
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG26560.1461172745167292No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA25630.14100096934728049No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24400.13423424315542895Clontech Universal Primer Mix Long (96% over 26bp)
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG24200.13313396247382706No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA23690.13032824673574228No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCG22790.12537698366853384No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22310.12273631003268935Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG20000.11002806816018766No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC18880.10386649634321717No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18530.10194100515041388No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)