FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001617154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001617154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2058169
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT85780.41677821403393017No Hit
ACATATGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC73750.3583282033691111Illumina Single End PCR Primer 1 (100% over 38bp)
ATATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC73200.35565592524229056Illumina Single End PCR Primer 1 (100% over 38bp)
ATATACGTATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG65460.3180496839666713Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATATACGTATATACGTATATACGAGATCGGAAGAGCGTCGTGTAGGG62840.3053199227079992Illumina Single End PCR Primer 1 (100% over 24bp)
ACGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC35970.17476698949406Illumina Single End PCR Primer 1 (100% over 40bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT33080.16072538260949418No Hit
ATATATACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC32820.15946212385863356Illumina Single End PCR Primer 1 (100% over 40bp)
GTATATACGTATATACGTATATACAGATCGGAAGAGCGTCGTGTAGGGAA32000.1554780001059194Illumina Single End PCR Primer 1 (100% over 26bp)
ATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC27710.13463423071671957Illumina Single End PCR Primer 1 (100% over 38bp)
ATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC26720.12982413008844268Illumina Single End PCR Primer 1 (100% over 40bp)
ATATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC25440.12360501008420591Illumina Single End PCR Primer 1 (100% over 40bp)
CGTATATACGTATATACGTATATACGTATATACGTATATACGTATATACG22860.11106959632566615No Hit
ATACATATGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA20700.1005748313185166Illumina Single End PCR Primer 1 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)