FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001617157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001617157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192114
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC153951.2914033389424167Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACATATGTATACATATGTATACATATGTATACATATGTATACATATGT35080.29426715901331585No Hit
ATGTATACATATGTATACATAGATCGGAAGAGCACACGTCTGAACTCCAG20450.17154399663119468Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACATATGTACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAG20320.17045349689710884Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATATGTATACATATGTATACATATGTATACATATGAGATCGGAAGAGCA19660.16491711363175No Hit
ATACATATGTATACATATGTATACATATGTATAGATCGGAAGAGCACACG18600.15602534656920394No Hit
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA17000.14260381138045522TruSeq Adapter, Index 8 (100% over 39bp)
ATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT16460.13807404325425254TruSeq Adapter, Index 8 (100% over 37bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA16010.13429923648241696TruSeq Adapter, Index 8 (100% over 39bp)
CATATGTATACATATGTATACATATGTATACATATGTATACATATGTATA14950.1254074694198709No Hit
ATACATATGTATACATATGTATACATATGTATACATATGTATAGATCGGA12480.10468797447224007No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)