FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001617163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001617163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597577
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACA98870.6188747083865128TruSeq Adapter, Index 2 (97% over 36bp)
ATATGTACATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACA88220.5522112549191682TruSeq Adapter, Index 2 (97% over 36bp)
ACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACA61610.38564651343878886TruSeq Adapter, Index 2 (97% over 36bp)
ACATATATGTACATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG56180.3516575413892413Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39830.24931505648867003No Hit
GTACATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACA25110.15717552268216178TruSeq Adapter, Index 2 (97% over 36bp)
ATATATGTACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACA22250.13927341217356035TruSeq Adapter, Index 2 (97% over 36bp)
GTACATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAAT16090.10071502030888026TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)