FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001617164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001617164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597577
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGTACATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC83040.5197871526693235Illumina Single End PCR Primer 1 (100% over 40bp)
ATGTACATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC75640.473467006598117Illumina Single End PCR Primer 1 (100% over 40bp)
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT65600.41062183544204756No Hit
ACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC53650.33582105901624776Illumina Single End PCR Primer 1 (100% over 40bp)
ACATATATGTACATATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAG50970.3190456547634324Illumina Single End PCR Primer 1 (100% over 30bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT43470.2720995607723446No Hit
ATATATGTACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC20200.12644147981599635Illumina Single End PCR Primer 1 (100% over 40bp)
GTACATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC18670.11686447664181444Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)