FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001617167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001617167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202715
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA242872.0193478920608787No Hit
ATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAA186101.5473324935666388TruSeq Adapter, Index 19 (100% over 39bp)
ATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAA170761.4197877302602862TruSeq Adapter, Index 19 (100% over 39bp)
ACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACG167511.3927655346445336TruSeq Adapter, Index 19 (97% over 40bp)
ATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACG145661.211093234889396TruSeq Adapter, Index 19 (97% over 40bp)
ACGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAA144281.1996191949048611TruSeq Adapter, Index 19 (100% over 39bp)
ATATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAA128821.0710766889911576TruSeq Adapter, Index 19 (100% over 39bp)
GTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT124591.0359062620820394TruSeq Adapter, Index 1 (97% over 35bp)
ATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGA113240.941536440470103TruSeq Adapter, Index 19 (100% over 37bp)
ATATACGTATATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT103010.8564788831934416TruSeq Adapter, Index 1 (97% over 35bp)
ATACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC95140.7910435971946803Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATACGTATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTC91850.7636888207098107Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACG64470.5360372157992542TruSeq Adapter, Index 19 (97% over 40bp)
ACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC64450.5358709253646957Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAG61520.5115093767018787Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATACGTATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC58160.4835725836960543Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGA54560.4536403054755283TruSeq Adapter, Index 19 (100% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT54330.45172796547810584TruSeq Adapter, Index 19 (97% over 40bp)
ATATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGA51120.42503835073147006TruSeq Adapter, Index 19 (100% over 37bp)
GTATATACGTATATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC47360.39377574903447615Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAC39170.3256798160827794TruSeq Adapter, Index 19 (100% over 40bp)
ATACATATGTATACATATGTATAGATCGGAAGAGCACACGTCTGAACTCC34280.2850218048332315Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAA32140.2672287283354743TruSeq Adapter, Index 19 (100% over 38bp)
ATATACGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAC31660.26323775790607085TruSeq Adapter, Index 19 (100% over 40bp)
GTATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGA27510.22873249273518667TruSeq Adapter, Index 19 (100% over 37bp)
ATATACGTATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAA26910.2237437796984323TruSeq Adapter, Index 19 (100% over 38bp)
ATACGTATATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGA25860.2150135318841122TruSeq Adapter, Index 19 (100% over 37bp)
ACATATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAA19860.16512640151656877TruSeq Adapter, Index 19 (100% over 39bp)
TACGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAA19650.16338035195370473TruSeq Adapter, Index 19 (100% over 38bp)
ATACATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAA17500.14550413023866834TruSeq Adapter, Index 19 (100% over 39bp)
ATATATACGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAA16580.13785477024897835TruSeq Adapter, Index 19 (100% over 38bp)
GTATATATACGTATATATACAGATCGGAAGAGCACACGTCTGAACTCCAG15940.13253347634310705Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT13910.11565499723542154TruSeq Adapter, Index 1 (97% over 35bp)
ATATACGTATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT13790.11465725462807067TruSeq Adapter, Index 1 (97% over 35bp)
ACGTATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGA13780.11457410941079142TruSeq Adapter, Index 19 (100% over 37bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13170.10950225115675784No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)