FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001617168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001617168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202715
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT211111.7552786819820156No Hit
ATATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC162041.3472851007927897Illumina Single End PCR Primer 1 (100% over 40bp)
ATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC150611.2522501174426195Illumina Single End PCR Primer 1 (100% over 40bp)
ATATACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG143691.194713627085386Illumina Single End PCR Primer 1 (100% over 42bp)
ACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG131651.0946067854811823Illumina Single End PCR Primer 1 (100% over 42bp)
ATATATACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC123731.0287557733960249Illumina Single End PCR Primer 1 (100% over 40bp)
ACGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC114460.9516801569781703Illumina Single End PCR Primer 1 (100% over 40bp)
ATATACGTATATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA103580.8612181605783581Illumina Single End PCR Primer 1 (100% over 36bp)
ATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC95950.7977783597942987Illumina Single End PCR Primer 1 (100% over 38bp)
GTATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA87620.7285183938006926Illumina Single End PCR Primer 1 (100% over 36bp)
ATATACGTATATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG81500.6776335208257983Illumina Single End PCR Primer 1 (100% over 32bp)
ATACGTATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG80880.6724785173544855Illumina Single End PCR Primer 1 (100% over 32bp)
ATATGGATCCGGCGCGCCGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT74740.6214273539450327No Hit
ATATACGTATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG72110.5995601618005928Illumina Single End PCR Primer 1 (100% over 30bp)
ATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG57570.4786670158765792Illumina Single End PCR Primer 1 (100% over 42bp)
ATATACGTATATACGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA53740.4468223976586307Illumina Single End PCR Primer 1 (100% over 34bp)
ACGTATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA50940.4235417368204437Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC46960.3904499403433066Illumina Single End PCR Primer 1 (100% over 38bp)
ATACGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC43390.36076709777461824Illumina Single End PCR Primer 1 (100% over 38bp)
GTATATACGTATATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA42400.3525357212639736Illumina Single End PCR Primer 1 (100% over 34bp)
ATACATATGTATACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAG37640.31295859783905583Illumina Single End PCR Primer 1 (100% over 28bp)
ATATACGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG35260.2931700361265969Illumina Single End PCR Primer 1 (100% over 41bp)
TACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG28620.23796161185318218Illumina Single End PCR Primer 1 (100% over 41bp)
ATATACGTATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT28330.23555040055208426Illumina Single End PCR Primer 1 (100% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27100.22532353882673783No Hit
TATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT23380.1943935179988609Illumina Single End PCR Primer 1 (100% over 39bp)
ATACGTATATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC22450.18666101279189168Illumina Single End PCR Primer 1 (100% over 38bp)
GTATATACGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC21520.17892850758492246Illumina Single End PCR Primer 1 (100% over 38bp)
ATATATACGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT17930.14907937458167564Illumina Single End PCR Primer 1 (100% over 39bp)
ATACATATGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC16910.14059856241919325Illumina Single End PCR Primer 1 (100% over 40bp)
ACATATGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC15450.12845936069642433Illumina Single End PCR Primer 1 (100% over 40bp)
GTATATATACGTATATATACAGATCGGAAGAGCGTCGTGTAGGGAAAGAG14490.12047741983761738Illumina Single End PCR Primer 1 (100% over 30bp)
TACGTATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT14320.11906395114387033Illumina Single End PCR Primer 1 (100% over 39bp)
ATATACGTATATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAG13700.1139089476725575Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATACGTATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA12050.10018998682148307Illumina Single End PCR Primer 1 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)