FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001617553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001617553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4537501
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471391.0388758041045059No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336990.7426775222749262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219810.4844296453047614No Hit
GTACATGGGGGATCAAGTGAATAAGTGCATATGGTGGATGCCTAGGCGAT195570.4310081694747836No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175810.38745996970579183No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135670.2989971792843682No Hit
GTATAAGACCGTACCGCAAACCGACACAGGTGGGTAGGATGAGAATTCTA132520.2920550320539874No Hit
GTACTGGTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGT128200.2825343729951795No Hit
CCTTTATACAATGCGATGTGGGGACGGAGAAGGTTAGGTCAGCCGGGTGT122110.2691128883497767No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119990.2644407130709172No Hit
GTATAAAGGTACAGGAATATTAACCTGTTTCCCATCAGCTACGCATCTCT117610.2591955351635184No Hit
GTCTTATACAACTGAAGCTTAGTGGCTTTTCTTGGAAGCTTGGTATCAAT97280.21439113732426726No Hit
GTGGTAGCCTGCGTAAAGTTTCGGGGAGCTGGCAAACAAGCTTTGATCCG95340.21011565617285816No Hit
TATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTAGCT92250.20330574031829415No Hit
ATATAAATATAGAGCATCCGAAGCTTCGGTTACGTGCTTAGCCCCGTTAC90020.19839114085043727No Hit
GTTATGACTAGGGGGATCATAAGATCTTACCAACTCATTGCAAACTCCGA82400.18159775612170664No Hit
CTCTATGGCCGGACTTTCCATTCCGTTCTACTATCGCATATGCTAAAAAT80360.17710188934393623No Hit
GAATAAGTGCATATGGTGGATGCCTAGGCGATTACAGGCGATGAAGGACG72750.16033054317784173No Hit
GTATAAGGGGCTATCACCCTCTATGGCCGGACTTTCCATTCCGTTCTACT71500.15757572284832555No Hit
AGTCATAACAGTGCTCTACCCCCGAGAATGATATACGAGGCACTACCTAA68700.1514049253102093No Hit
AGTCTATACACACTACCAGGGCGCACCTTCTCCCGAAGTTACGGTGCTAA67460.14867214354332925No Hit
GTGCATATGGTGGATGCCTAGGCGATTACAGGCGATGAAGGACGTGGTAG67230.14816525660269828No Hit
ATATTTATATAGATGCATGGTAGGAGAGCGTTCTGTAGGCCTGCGAAGGT66760.14712944415880017No Hit
CCCTAGTTCCACACACGGGATTTCGTATAAGGGGCTATCACCCTCTATGG66390.1463140173412634No Hit
GCCTTGGAGGATGGTCCCCCCATGTTCAGACAAGGTTTCTCGTGCCCCGC65420.14417627676555886No Hit
GTTCCATGTTCGCTCGCCACTACTTACGGAATCTCGGTTGATTTCTTTTC65390.14411016107765046No Hit
GTATAGACTTGCTCTAGAAGCACGATAGGGTTGCAGTGAAAAGGTGGCTG64630.14243523031730462No Hit
GTTCACTATCGGTCGATTACGAGTATTTAGCCTTGGAGGATGGTCCCCCC64490.1421266904403988No Hit
CTATAATACCATGCTAATATATTCAAAGCTAAAAAGCTTGTTTTGAACAC64350.14181815056349298No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTATTAAACA62790.1383801347922568No Hit
GTGAATAAGTGCATATGGTGGATGCCTAGGCGATTACAGGCGATGAAGGA60970.13436911639248125No Hit
ATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTAGCTC60730.13384019088921414No Hit
GTGGATGCCTAGGCGATTACAGGCGATGAAGGACGTGGTAGCCTGCGTAA55860.12310741088541909No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC55780.12293110238433004No Hit
GAATATATAGGGAGGTTGTGGCAAACCGAGTGAACTGAAACATCTAAGTA54380.11984570361527193No Hit
CTACCAGGGCGCACCTTCTCCCGAAGTTACGGTGCTAATTTGCCGAGTTC54250.11955920230100225No Hit
GTACTAGTTCACTATCGGTCAGTCAGTAGTATTTAGCCTTGGAGGATGGT53070.11695865190993898No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53050.11691457478466671No Hit
ATCCACAGCTCATCCCCAAATTTTTCAACATTTGTGGGTTCGGACCTCCA51380.11323413482443309No Hit
GCATATGGTGGATGCCTAGGCGATTACAGGCGATGAAGGACGTGGTAGCC49620.10935534780047432No Hit
CTACTATCGCATATGCTAAAAATAACAGGCTGTTCCATGTTCGCTCGCCA49470.10902476936093237No Hit
ACATTATGCTGAGGCGTGATAACGAGTCTACTTGTAGACGAAGTGATTGA47940.10565286927760456No Hit
GTATCAACGCAGAGTACATGGGGGATCAAGTGAATAAGTGCATATGGTGG47620.10494763527324842No Hit
CATCTAAGTAGCTCGAGGAAAAGAAATCAACCGAGATTCCGTAAGTAGTG47390.10444074833261745No Hit
TCGTTACGCCATTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCT47220.10406609276780324No Hit
GTAGGAGAGCGTTCTGTAGGCCTGCGAAGGTGTTCTGTGAGGGATGCTGG46990.10355920582717226No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT46600.10269970188436321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)