FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001617721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001617721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2655973
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAG1114754.19714357036009Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC810413.0512734880964527Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATACGTATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTC766032.884178415970343Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGA660032.4850779733077104TruSeq Adapter, Index 21 (97% over 40bp)
ATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGA638192.4028482217251455TruSeq Adapter, Index 21 (97% over 40bp)
ATATACGTATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC563222.1205787860042253Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC552712.0810076006043734Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCG503591.896065961513916TruSeq Adapter, Index 21 (100% over 39bp)
ATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCG482071.8150410414563702TruSeq Adapter, Index 21 (100% over 39bp)
GTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT446251.680175212624526TruSeq Adapter, Index 1 (97% over 35bp)
ATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGA424541.598434923849TruSeq Adapter, Index 21 (97% over 40bp)
ATATACGTATATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT402041.5137202072460827TruSeq Adapter, Index 1 (97% over 35bp)
GTATATACGTATATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC398611.5008059193372825Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT391861.4753915043564072TruSeq Adapter, Index 21 (100% over 37bp)
CGTATATACGTATATACGTATATACGAGATCGGAAGAGCACACGTCTGAA351621.3238839400852342Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTATATACGTATATACGTATATACAGATCGGAAGAGCACACGTCTGAACT207860.7826133774703281Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATACGTATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC144430.5437931786204152Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATACGTATATACGTATATACGTATATAGATCGGAAGAGCACACGTCTG117940.4440557189399139Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGG106780.4020372195048669TruSeq Adapter, Index 21 (100% over 40bp)
CGTATATACGTATATACGTATATACGTATATACGAGATCGGAAGAGCACA104000.3915702456312621No Hit
ATATACGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGG100110.3769240124052466TruSeq Adapter, Index 21 (100% over 40bp)
CGTATATACGTATATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC96050.3616377124315646Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGTATATACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAA95900.36107294765421183Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATACGTATATACGTATATACGTATAGATCGGAAGAGCACACGTCTGAA93210.35094483264701865Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTC92340.3476691969383725TruSeq Adapter, Index 21 (100% over 38bp)
ATATACGTATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTC88830.3344537011483174TruSeq Adapter, Index 21 (100% over 38bp)
ATACGTATATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT82570.31088418444012794TruSeq Adapter, Index 21 (100% over 37bp)
GTATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT79690.3000407007149546TruSeq Adapter, Index 21 (100% over 37bp)
CGTATATACGTATATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA77830.2930376174757801Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTATATACGTATATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA75930.28588393029597814Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATACGTATATACGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA72080.2713883010105901Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA71910.2707482342629236Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATACGTATATACGTATATACGTATAGATCGGAAGAGCACACGTCTGAACT55500.20896296762052927Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG47190.17767499895518518Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGTATATACGTATATACAGATCGGAAGAGCACACGTCTGAACTCCAGTC44960.16927882926520713Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTATATACGTATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC44560.16777278985893304Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTATATACGTATATACGTATATACGTATATACAGATCGGAAGAGCACACG43140.16242634996666006No Hit
ATACGTATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC42980.16182393420415042Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATACGTATATACGTATATACGTAGATCGGAAGAGCACACGTCTGAACT42840.16129682041195448Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGTATATACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACT42000.15813413765877893Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATACGTATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC41220.15519736081654445Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGTATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT39690.14943676008754606TruSeq Adapter, Index 1 (97% over 35bp)
TATATACGTATATACGTATATAAGATCGGAAGAGCACACGTCTGAACTCC39330.14808132462189938Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATACGTATATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG38470.1448433398984101Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGTATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT33840.12741093377078758TruSeq Adapter, Index 1 (97% over 35bp)
TATATACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCA33830.12737328278563073Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATATACGTATATACGTATATACGTATATACGTATATACGTATATACGTAT33740.12703442391921907No Hit
TATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT33720.12695912194890535TruSeq Adapter, Index 9 (97% over 36bp)
ATATACGTATATACGTATATAAGATCGGAAGAGCACACGTCTGAACTCCA33330.1254907335277881Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATATACGTATATACGTATATACGTATATACGTATATACGTATATACGAAT31860.11995603870973086No Hit
CGTATATACGTATATACGTATATACGTATATACGTATATACGTATATACG31550.11878885816986845No Hit
ATATACGTATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT30220.11378127714400711TruSeq Adapter, Index 9 (97% over 36bp)
ATACGTATATATACGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC28690.10802067641500875Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATACGTATATACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG28550.10749356262281282Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGTATATATACGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC28430.10704175080093058Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTATATACGTATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG28030.10553571139465649Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATACGTATATACGTATATACAGATCGGAAGAGCACACGTCTGAACTCC27430.10327665228524537Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTATATACGTATATACGTATATAGATCGGAAGAGCACACGTCTGAACTCC26880.10120584810161849Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)